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目的:研究过量人参皂苷对原代培养小鼠肝细胞的毒性作用。方法:小鼠原代肝细胞培养液中加入人参皂苷(终浓度分别为4000 mg/L,200 mg/L和10 mg/L),设为高、中、低浓度组,台盼蓝拒染法及MTT法测定人参皂苷对肝细胞增殖的影响,并测定肝细胞培养液中谷丙转氨酶(GPT)及谷草转氨酶(GOT)的含量。结果:与对照组比较,高浓度人参皂苷对原代培养肝细胞增殖有明显的抑制作用,给药后3 d、4 d、5 d、6 d和7 d,高浓度组活细胞数分别为对照组的85%、81%、71%、64%和59%。人参皂苷给药24 h开始,高浓度组培养液上清中GPT含量均显著升高(P<0.05);给药48 h、72 h和96 h时高浓度组细胞培养液GPT含量分别为对照组的2.6倍、8.0倍和12.5倍(P<0.01);随着给药时间延长,GPT含量呈上升趋势。给药72 h和96 h后高浓度组细胞与空白组比较,GOT含量均显著增加(P<0.05)。结论:过量人参皂苷能抑制肝细胞增殖,引起肝细胞损伤,对肝细胞可能存在直接的毒副作用。
Objective: To study the toxic effects of excessive ginsenosides on primary cultured mouse hepatocytes. Methods: Ginsenosides (4000 mg / L, 200 mg / L and 10 mg / L, respectively) were added to the primary hepatocyte culture medium of mice to set high, medium and low concentrations of trypan blue exclusion Method and MTT assay of ginsenosides on the proliferation of hepatocytes, and determination of the content of alanine aminotransferase (GPT) and aspartate aminotransferase (GOT) in the hepatocyte culture medium. Results: Compared with the control group, high concentrations of ginsenoside significantly inhibited the proliferation of primary cultured hepatocytes. The numbers of viable cells in high concentration group at 3 d, 4 d, 5 d, 6 d and 7 d after administration were Control groups 85%, 81%, 71%, 64% and 59%. GPT content in the supernatant of the high concentration group was significantly increased at 24 h after administration of ginsenoside (P <0.05). The GPT levels of the cell culture medium in the high concentration group for 48 h, 72 h and 96 h were Group were 2.6 times, 8.0 times and 12.5 times (P <0.01). With the prolongation of administration time, GPT content increased. After 72 h and 96 h, GOT content increased significantly in high concentration group compared with blank group (P <0.05). Conclusion: Excessive ginsenosides can inhibit the proliferation of hepatocytes and cause damage to hepatocytes, which may have direct toxic and side effects on hepatocytes.