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目的观察凋亡抑制蛋白Livin在肾癌组织内的表达情况,分析Livin的表达与肾癌临床病理特征之间的关系。方法应用免疫组化法和Westernblot技术观察Livin在肾癌组织内的表达。结果 Livin在肾癌组织及正常肾组织中均有表达,Livin蛋白表达于肿瘤细胞胞质,在48例肾癌组织中Livin阳性表达率为79.2%,对照组16例正常肾组织中Livin阳性表达率为18.8%,二者之间差别具有统计学意义(<0.05)。Westernblot检测结果表明在肾癌组织中Livin蛋白的表达量显著高于正常肾组织的表达水平(<0.05)。Livin表达与患者性别、年龄、肿瘤分化程度无关,与临床分期和淋巴结转移显著相关(<0.05)。Kaplan-Meier生存分析结果表明,Livin阴性患者无瘤生存率明显高于Livin阳性患者(<0.05)。结论 Livin在肾癌组织高表达与肾癌的进展和淋巴结转移密切关系,可能预示肾癌复发。
Objective To observe the expression of Livin in renal cell carcinoma and to analyze the relationship between the expression of Livin and clinicopathological features of renal cell carcinoma. Methods Immunohistochemistry and Western blotting were used to observe the expression of Livin in renal cell carcinoma. Results Livin was expressed in both renal cell carcinoma and normal renal tissue, and Livin protein was expressed in the cytoplasm of tumor cells. The positive rate of Livin in 48 cases of renal cell carcinoma was 79.2%, while that in 16 cases of normal renal tissue in control group The rate was 18.8%, the difference between the two was statistically significant (<0.05). The results of Western blot showed that the expression of Livin in renal cell carcinoma was significantly higher than that in normal renal tissues (<0.05). The expression of Livin was not related to the patient’s sex, age, tumor differentiation degree, and was significantly associated with clinical stage and lymph node metastasis (<0.05). Kaplan-Meier survival analysis showed that the tumor-free survival rate of Livin-negative patients was significantly higher than that of Livin-positive patients (<0.05). Conclusion The high expression of Livin in renal cell carcinoma is closely related to the progress of renal cell carcinoma and lymph node metastasis, which may indicate the recurrence of renal cell carcinoma.