Objective: To compare the plasma folate level, prevalence of folate deficiency and the related influencing factors of hypertension in Xinjiang agricultural and pastoral areas. Methods: We enrolled 1926 study subjects with 447 hypertensive and non-hypertensive 1479 ones aged ≥15 years old using multi-stage stratified sampling in Emin Xinjiang between January and December 2014, conducted the health behavior questionnaire and physical examination, and collected blood samples. The plasma folate level were measured by chemiluminescence method. Results: (1) There were significant differences (P<0.05) in age, gender, ethnic composition, marital status, education level, smoking consumption, drinking data, overweight and obesity and hyperhomocysteinemia (HHcy) between hypertension group and non hypertension group. (2) The folate deficiency rate of hypertension group was higher than that of non hypertension group (P=0.003); the folate deficiency rate of systolic and diastolic hypertension was the highest (P=0.008); the folate level of hypertension with HHcy was the lowest (P<0.001) and the folate deficiency rate was the highest (P=0.023). (3) Partial correlation analysis showed that folate was negatively correlated with systolic blood pressure and pulse pressure (P<0.05). (4) Multivariate logistic regression analysis showed that age 45-59 (OR=3.78, 95％ CI: 2.45-5.82), age≥60 (OR=6.87, 95％ CI:4.35-5.10.87), male gender (OR=6.96, 95％ CI: 3.86-12.54), Kazakhs (OR=2.12, 95％ CI:1.52-2.96), Mongolian (OR=2.38, 95％ CI: 1.25-4.53), smoking (OR=4.21, 95％ CI:2.05-8.63), drinking (OR=6.36, 36％ CI: 3.00-13.48), overweight and obesity (OR=2.11, 95％ CI: 1.58-2.81), and folate deficiency (OR=1.72, 95％ CI: 1.05-2.80) were the related factors of hypertension. Conclusions: The prevalence of folate deficiency in the population with hypertension in Xinjiang is higher than that in the non hypertension group, and the highest prevalence of folate deficiency is in hypertension with HHcy. Therefore, folate supplementation in the prevention of hypertension may be helpful, especially for the elderly, men and Kazakhs, and at the same time, healthy life style has an important reference value for reducing the level of hypertension and preventing and controlling cardiovascular diseases.