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汞是重要的污染元素之一,尤以有机汞的毒性更大.早期的研究工作,大多采用气相色谱法,它要求试样必须具有挥发性和热稳定性,因而在应用上受到一定限制.近年来有关高效液相色谱分析的报道逐渐增多,柱前衍生和柱中衍生化技术均有应用,常用的衍生化试剂有APDC、DDTC和双硫腙等. 铋试剂Ⅱ是一种性能优良的广谱性螯合剂,对汞有良好的络合性能.我们将它用作有机汞的柱前衍生化试剂,在C_8柱上以正丁醇-甲醇-水作流动相,苹果酸为辅助络合剂,分离和定量测定了有机汞的3种形态,检测限分别为1.4ng(MeHg~+)、1.0ng(EtHg~+)和2.5ng(PhHg~+),并成功地应用于东湖水样的分析.
Mercury is one of the most important pollutants, especially organic mercury is more toxic.Most of the early research work, the use of gas chromatography, which requires the sample must be volatile and thermal stability, and therefore be subject to certain restrictions. In recent years, reports of high performance liquid chromatography have been gradually increasing. Pre-column derivatization and column derivatization have been used. Common derivatizing reagents are APDC, DDTC and dithizone etc. Bismuth reagent II is an excellent The broad-spectrum chelating agent has good complexability to mercury.We use it as a pre-column derivatization reagent for organic mercury, using n-butanol-methanol-water as the mobile phase and malic acid as the auxiliary phase on the C_8 column The detection limits were 1.4ng (MeHg ~ +), 1.0ng (EtHg ~ +) and 2.5ng (PhHg ~ +), respectively, and were successfully applied to Donghu water Kind of analysis.