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目的:建立HPLC法同时测定白英叶中绿原酸、咖啡酸及芦丁含量的方法。方法:采用HPLC法,Angilent ZorbaxODS C_(18)色谱柱(250 mm×4.6 mm,5μm),柱前加保护柱;流动相为乙腈-1%冰醋酸溶液,梯度洗脱;流速:0.6 ml·min~(-1);柱温:25℃;检测波长327 nm。结果:绿原酸质量浓度在2.16～43.20μg.·ml~(-1)(r=0.999 5)内与峰面积呈良好的线性关系,平均回收率为99.05%,RSD=1.29%;咖啡酸质量浓度在2.15～43.04μg·ml~(-1)(r=0.999 8)内与峰面积呈良好的线性关系,平均回收率为100.04%,RSD=1.65%;芦丁质量浓度在5.24～104.74μg·ml~(-1)内与峰面积呈良好的线性关系,平均回收率99.60%,RSD=1.74%。结论:该方法准确、重复性良好,能同时测定白英叶中的3种有效成分,可用于白英药材及饮片的质量控制。
Objective: To establish a HPLC method for the simultaneous determination of chlorogenic acid, caffeic acid and rutin in Cotinus coggygria leaves. Methods: The column was packed with Angilent Zorbax ODS C 18 column (250 mm × 4.6 mm, 5 μm) by HPLC. The mobile phase consisted of acetonitrile-1% glacial acetic acid with gradient elution. The flow rate was 0.6 ml · min ~ (-1); column temperature: 25 ℃; detection wavelength 327 nm. Results: The concentration of chlorogenic acid in the range of 2.16 ~ 43.20μg · ml ~ (-1) (r = 0.999 5) showed a good linear relationship with the peak area. The average recovery was 99.05%, RSD was 1.29% The linear range of the mass concentration was 2.15 ~ 43.04μg · ml -1 (r = 0.999 8) with the average recovery of 100.04% and the RSD of 1.65%. The mass concentration of rutin was 5.24 ~ 104.74 There was a good linear relationship between peak area and μg · ml -1, with an average recovery of 99.60% and RSD of 1.74%. Conclusion: The method is accurate and reproducible. The method can simultaneously determine the three active ingredients in the leaves of Cinnamomum villosum and can be used for the quality control of Crab White Clover and Pieces.