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目的 大量的研究证据表明 ,叙利亚仓鼠胚胎 (SHE)细胞转化试验可能是目前用于检测致癌物和研究化学致癌机制的试验中生物相关性最强短期试验。本研究旨在检测来源于马兜铃鼠属类草药中的主要活性成分马兜铃酸 (AA)引起SHE细胞形态学转化的可能性和抗氧化剂α 生育酚对AA所诱导的SHE细胞形态学转化的影响 ,并以此为例来说明SHE细胞转化试验可以用于检测营养保健品和中草药中可能存在的致癌或抗癌成分。方法 在进行正式的细胞形态学转化试验之前 ,先进行了初步的剂量范围选择试验 ,以确定用于形态学转化试验的AA的浓度。剂量范围选择试验是在 2 4h染毒或 7d连续性染毒的两种条件下进行的。根据剂量范围选择试验的结果 ,那些引起 0 %～ 5 0 %细胞毒性的剂量被用于 2 4h染毒或 7d连续性染毒的细胞形态学转化试验来检测AA引起SHE细胞形态学转化的可能性。至于α 生育酚对AA所诱导的SHE细胞形态学转化的影响 ,则使用了用可引起SHE细胞形态学转化浓度的AA和 10 0 μmol·L- 1α 生育酚同时处理SHE细胞的方法进行了观察。结果 在 7d连续性染毒的条件下 ,0 .4 ,0 .8以及 1.6mg·L- 1的AA引起了SHE细胞形态学转化率的显著性升高 ;在 2 4h染毒的条件下 ,1.0 ,1.5 ,2 .5 ,4 .5和 5 .0mg·L- 1等5个剂量组的形态学
OBJECTIVE: A large amount of research evidence indicates that the Syrian hamster embryo (SHE) cell transformation assay may be the most biologically relevant short-term trial currently used for the detection of carcinogens and the study of chemical carcinogenesis mechanisms. The aim of this study was to examine the possibility of morphological transformation of SHE cells induced by the aristolochic acid (AA), the main active ingredient in the herb of the species Aristolochia and antioxidants α-tocopherol to AA-induced SHE cell morphology. The effect of the transformation was used as an example to illustrate that the SHE cell transformation assay can be used to detect possible carcinogenic or anticancer components in nutraceuticals and Chinese herbal medicines. METHODS Prior to conducting a formal cell morphology transformation assay, a preliminary dose range selection test was performed to determine the concentration of AA used in the morphological transformation assay. The dose range selection test was performed under two conditions of 24 h exposure or 7 d continuous exposure. According to the results of the dose range selection test, those doses that caused 0% to 50% cytotoxicity were used in a 24 h cytotoxicity or 7 d continuous exposure cell morphology transformation assay to detect the possible morphological transformation of SHE cells. Sex. As for the effect of alpha tocopherol on the morphological transformation of SHE cells induced by AA, the method of simultaneous treatment of SHE cells with AA and 100 μmol·L - 1α tocopherol, which can cause the morphological transformation concentration of SHE cells, was used. . Results Under the condition of continuous exposure for 7 days, the AA of 0.4, 0.8, and 1.6 mg·L -1 caused a significant increase in the morphological conversion rate of SHE cells; under the condition of 24 hours exposure, Morphology of 1.0, 1.5, 2.5, 4.5, and 5.0 mg·L -1 five-dose groups