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目的 研究中药虎杖煎剂在治疗大鼠肝脏缺血性损伤后肝组织的病理学改变,证实该药对急性肝脏缺血性损伤有治疗作用。 方法 建立大鼠常温下肝门完全阻断的模型,观察肝脏缺血损伤后虎杖组和普食组在不同的时间段肝组织的病理学改变。 结果 通过光镜和电镜发现,术后1d普食组与虎杖组肝细胞肿胀,结构破坏,肝窦内皮细胞孔隙加大,内皮破坏,内皮之间可见孔道。术后4d普食组肝小叶结构仍破坏,线粒体肿胀,颗粒变性,而虎杖组未见肝细胞坏死改变,细胞膜特化部分如桥粒、毛细胆管区微绒毛有轻微破坏。术后7d普食组肝细胞变性仍可见,线粒体轻度肿胀,基质变化,膜结构欠清楚,粗面内质网欠规则,而虎杖组肝细胞基本恢复正常形态。 结论 虎杖煎剂具有改善损伤肝组织的微循环,抑制白细胞、血小板与肝脏内皮细胞的粘附,达到促进肝细胞再生、修复损伤的能力,为临床上肝脏外科围手术期的应用奠定了病理学基础。
Objective To study the pathological changes of hepatic tissue after treatment of hepatic ischemic insults of Polygonum cuspidatum decoction, and to prove that the drug has therapeutic effects on acute ischemic hepatic injury. Methods To establish a rat model of hepatic occlusion completely at room temperature. Observe the pathological changes of hepatic tissue at different time points after the liver ischemic injury. Results Through light microscopy and electron microscopy, it was found that hepatocyte swelling and structural destruction in the normal food group and Polygonum cuspidatum group occurred one day after surgery, and the pores of the sinusoidal endothelial cells were enlarged, the endothelium was destroyed, and the pores were seen between the endothelium. After 4 days, the structure of hepatic lobule in normal diet was still damaged, mitochondria were swollen, and the granules were denatured. However, there was no necrosis of hepatocytes in the Polygonum cuspidatum group. There were slight destruction of the microvilli in the desmosomes such as desmosomes and bile ducts. At 7 days after operation, hepatocyte degeneration was still observed in the normal food group. Mild mitochondria were swollen, the matrix was changed, the membrane structure was unclear, the rough endoplasmic reticulum was less regular, and the liver cells of the Polygonum cuspidatum group basically returned to normal morphology. Conclusion Polygonum cuspidatum decoction can improve the microcirculation of the injured liver tissue, inhibit the adhesion of leukocytes, platelets and liver endothelial cells, and can promote the regeneration of hepatocytes and repair the damage. It has laid the pathology for the clinical application of liver surgery in the perioperative period. basis.