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本研究探讨了CALR基因对细胞生长和凋亡的影响,以MDS患者的肿瘤细胞为研究对象,构建CALR-RNAi慢病毒载体,观察其对SKM-1细胞生长和凋亡的影响。结果证实,MDS患者CALR基因表达较对照组明显降低(p<0.05)。成功构建的3种CALR-RNAi慢病毒载体中,CALR-RNAi(3)慢病毒载体敲除效率最高,转染SKM-1后可促进细胞生长、抑制细胞凋亡(p<0.05),同时CALR-RNAi(3)组的G0/G1期细胞减少,S期细胞增多。CALR基因在MDS的发病及发展中扮演着抑癌基因的角色,其突变及缺失是患者发病的主要原因,关于本基因序列的深入研究对于MDS的临床治疗有重大意义。
In this study, the effects of CALR gene on cell growth and apoptosis were investigated. The tumor cells of MDS patients were studied to construct CALR-RNAi lentiviral vector and the effect of CALR on the growth and apoptosis of SKM-1 cells was observed. The results confirmed that CALR gene expression in MDS patients was significantly lower than the control group (p <0.05). Among the three CALR-RNAi lentivirus vectors successfully constructed, CALR-RNAi (3) lentiviral vector knocked out the most efficiently, and SKM-1 transfected cells could promote cell growth and inhibit apoptosis (p <0.05) -RNAi (3) group of G0 / G1 phase cells decreased, S phase cells increased. CALR gene plays a role of tumor suppressor gene in the pathogenesis and development of MDS. The mutation and deletion of CALR gene are the main reasons for the onset of the disease. The in-depth study of this gene sequence is of great significance for the clinical treatment of MDS.