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【目的】探讨单克隆抗体 (McAB)的F(ab′) 2 片段在卵巢癌放射免疫显像诊断中的应用价值。【方法】利用胃蛋白酶消化抗癌胚抗原单克隆抗体 (C50 ) ,酶切产物经SDS PAGE电泳鉴定后 ,经SephadexG 10 0凝胶柱纯化 ,获得F(ab′) 2 片段。分别将99mTc标记的C50 ,F(ab′) 2 及正常鼠IgG行荷人卵巢癌裸鼠移植瘤放免显像 ,并比较其结果。【结果】①片段F(ab′) 2 的产率为 33%± 3 2 % ,其最大理论产率的 49%。酶联免疫吸附方法和免疫细胞化学染色均证实其保留较好的免疫活性。② 2 4h放射免疫显像可见片段组移植瘤部位有较高浓度的放射性物质聚集 ,肿瘤与非肿瘤组织放射性活度之比 (T/NT)片段组最高。【结论】利用单克隆抗体片段进行放免显像 ,效果优于全抗体。
【Objective】 To investigate the value of F (ab ’) 2 fragment of monoclonal antibody (McAb) in the diagnosis of ovarian cancer by radioimmunoimaging. 【Method】 The monoclonal antibody against carcinoembryonic antigen (C50) was digested by pepsin. The digested product was identified by SDS PAGE electrophoresis and purified by SephadexG 10 0 gel column to obtain F (ab ’) 2 fragment. The 99mTc-labeled C50, F (ab ’) 2 and normal murine xenografts of human ovarian cancer xenografts in nude mice were studied by radioimmunoassay and the results were compared. 【Result】 ① The yield of fragment F (ab ’) 2 was 33% ± 32%, which was 49% of the maximum theoretical yield. Enzyme-linked immunosorbent assay and immunocytochemical staining confirmed its retention of better immunocompetence. (2) Radioimmunoimaging 24 h radioactive immunohistochemical imaging showed that there was a higher concentration of radioactive material in the site of tumor xenografts, and the ratio of tumor activity to non-tumor tissue radioactivity (T / NT) was the highest. 【Conclusion】 The use of monoclonal antibody fragments for radioimmunoimaging, the effect is better than the whole antibody.