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为研究灵芝多糖(GLB7) 对小鼠腹腔巨噬细胞( MΦ) 三磷酸肌醇(IP3) 和二酰基甘油(DAG) 的作用,确定其对MΦ信号转导途径,采用细胞培养、放射免疫分析及离子交换层析和薄层层析法测定了小鼠腹腔MΦ中IP3 和DAG 的变化。结果显示GLB7 能引起小鼠MΦ中IP3 和DAG 浓度升高。IP3 的达峰时间为30s,百日咳毒素(PTX) 对此现象有抑制作用;DAG 的合成出现两个峰,第一个峰迅速短暂,峰值位于30s,第二个峰是持续近2min 的迟发峰。表明IP3/Ca2 + 和DAG/PKC 两条信息途径均参与了灵芝多糖对MΦ的免疫调节
To investigate the effects of GLB7 on murine peritoneal macrophages (MΦ) inositol trisphosphate (IP3) and diacylglycerol (DAG), to determine its signal transduction pathway for MΦ, using cell culture and radioimmunoassay The changes of IP3 and DAG in the peritoneal MΦ of mice were measured by ion exchange chromatography and thin layer chromatography. The results show that GLB7 can cause increased concentrations of IP3 and DAG in mouse MΦ. The peak time of IP3 was 30 s. Pertussis toxin (PTX) had an inhibitory effect on this phenomenon; the synthesis of DAG showed two peaks. The first peak was rapidly transient, the peak was at 30 s, and the second peak was delayed for nearly 2 min. peak. It was shown that both IP3/Ca2+ and DAG/PKC pathways were involved in the immunomodulation of Ganoderma lucidum polysaccharides on MΦ