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为研究灵芝多糖（ＧＬＢ７） 对小鼠腹腔巨噬细胞（ ＭΦ） 三磷酸肌醇（ＩＰ３） 和二酰基甘油（ＤＡＧ） 的作用，确定其对ＭΦ信号转导途径，采用细胞培养、放射免疫分析及离子交换层析和薄层层析法测定了小鼠腹腔ＭΦ中ＩＰ３ 和ＤＡＧ 的变化。结果显示ＧＬＢ７ 能引起小鼠ＭΦ中ＩＰ３ 和ＤＡＧ 浓度升高。ＩＰ３ 的达峰时间为３０ｓ，百日咳毒素（ＰＴＸ） 对此现象有抑制作用；ＤＡＧ 的合成出现两个峰，第一个峰迅速短暂，峰值位于３０ｓ，第二个峰是持续近２ｍｉｎ 的迟发峰。表明ＩＰ３／Ｃａ２ ＋ 和ＤＡＧ／ＰＫＣ 两条信息途径均参与了灵芝多糖对ＭΦ的免疫调节
To investigate the effects of GLB7 on murine peritoneal macrophages (MΦ) inositol trisphosphate (IP3) and diacylglycerol (DAG), to determine its signal transduction pathway for MΦ, using cell culture and radioimmunoassay The changes of IP3 and DAG in the peritoneal MΦ of mice were measured by ion exchange chromatography and thin layer chromatography. The results show that GLB7 can cause increased concentrations of IP3 and DAG in mouse MΦ. The peak time of IP3 was 30 s. Pertussis toxin (PTX) had an inhibitory effect on this phenomenon; the synthesis of DAG showed two peaks. The first peak was rapidly transient, the peak was at 30 s, and the second peak was delayed for nearly 2 min. peak. It was shown that both IP3/Ca2+ and DAG/PKC pathways were involved in the immunomodulation of Ganoderma lucidum polysaccharides on MΦ