论文部分内容阅读
将被黄瓜花叶病毒CMV-BF株系侵染的三生烟(Nicotiana tabacum var.Samsun NN)的不同单株分成两组分别置于高温(30±5℃)和低温(10±5℃)条件下,观察和分析CMV-BF侵染30、80 d时植株的症状表现及CMV2b基因的序列变异规律。结果表明:侵染30d时,在高温环境中的植株表现出畸形叶片较多、病毒症状较严重的现象,而低温培养的植株细弱,部分叶片大面积白化;2b基因DNA序列的多态性也以高温处理组略高于低温组,但差异未达到显著水平。侵染80 d时,高温组植株的病毒症状有所恢复,畸形叶片减少,而低温组植株除白化现象更严重外,畸形叶片增多;2b基因的多态性以低温处理组明显高于高温组,且达到显著水平(P<0.05)。因此,长时间低温处理有助于加强CMV-BF2b基因的多态性,而且2b基因的多态性可能与植株的病毒症状表现相关。
The different plants of Nicotiana tabacum var.Samsun NN infected with cucumber mosaic virus CMV-BF were divided into two groups, which were respectively placed in high temperature (30 ± 5 ℃) and low temperature (10 ± 5 ℃) Under the conditions of the CMV-BF infection 30 and 80 d observed the symptoms and CMV2b gene sequence variation. The results showed that: at 30 days after inoculation, the plants in high temperature environment showed more malformed leaves and more severe virus symptoms, whereas the plants in low temperature were thin and some of the leaves were whitened altogether. The polymorphism of 2b gene DNA sequence At the high temperature treatment group slightly higher than the low temperature group, but the difference did not reach a significant level. At 80 days after infection, the virus symptoms of the high-temperature group plants recovered, the deformed leaves reduced, while the low-temperature plants had more whitening phenomenon than that of the low-temperature group. The polymorphism of 2b gene in the low-temperature treatment group was significantly higher than that in the high-temperature group , Reaching a significant level (P <0.05). Therefore, prolonged hypothermia can enhance the polymorphism of CMV-BF2b gene, and the polymorphism of 2b gene may be related to the performance of the virus in the plant.