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目的研究脑出血模型血肿周围组织雌激素受体表达变化,以初步探索其是否参与脑出血后的神经保护作用。方法立体定位注射自体尾血建立小鼠尾状壳核出血模型,利用免疫组化、Western blot检测造模3 d后血肿周围组织ERs表达情况,测量、统计其光密度值。结果 ICH 3 d后,尼氏染色见血肿周围组织神经元萎缩、密度降低;免疫组化、Western blot均显示血肿周围组织ERs表达显著高于血肿对侧正常组织(P<0.05,P<0.01)。结论 ERs在ICH后表达上调,提示其可能介导雌激素的神经保护作用而影响ICH继发病理损害。
Objective To study the changes of estrogen receptor (ER) expression in the perihematomal brain tissue of intracerebral hemorrhage (ICH) model and to explore the neuroprotective effect after intracerebral hemorrhage. Methods The caudate putamen model was established by stereotactic injection of autologous tail blood to detect the expression of ERs in the perihematoma tissue around the hematoma by immunohistochemistry and Western blot. Results After 3 hours of ICH, neuronal atrophy and densification of perihematoma tissue were observed by Nissl staining. The expression of ERs in perihematoma tissue was significantly higher than that in hematoma (P <0.05, P <0.01) by immunohistochemistry and Western blot, . Conclusion The expression of ERs is up-regulated after ICH, suggesting that ERs may mediate neuroprotection of estrogen and may affect the secondary pathological damage of ICH.