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本文将合成的直径为10nm的正电荷金纳米颗粒通过静电作用高密度自组装到带负电荷的长链λ-DNA分子上,形成了高密度的具有纳米间隙的金纳米颗粒网络结构。研究了孤立的金纳米颗粒和所自组装的金纳米颗粒-DNA复合材料作为表面增强拉曼散射(SERS)基底的活性。原本对SERS信号响应较弱的10nm直径的金纳米颗粒,在自组装到DNA上形成具有纳米间隙的金纳米颗粒网络后,产生了均匀、一致、强烈的SERS增强响应。我们利用用该基底对罗丹明G(R6G)、吡啶(Py)和对巯基苯胺(4-ATP)等不同类型的小分子化合物进行SERS检测的结果表明,此方法制备SERS基底产率高、均一,具有较好的SERS增强效果好,SERS信号稳定性和重复性相对常规孤立的金纳米颗粒SERS基底有很大提高。
In this paper, the gold nanoparticles with the diameter of 10nm synthesized by high-density electrostatic self-assembly to the negatively charged long-chain λ-DNA molecules, forming a high-density nano-gap nano-particles network structure. Isolated gold nanoparticles and self-assembled gold nanoparticle-DNA composites were investigated as surface-enhanced Raman scattering (SERS) substrate activity. The 10nm-diameter gold nanoparticles, which originally responded weakly to the SERS signal, produced a uniform, consistent and strong SERS-enhanced response after self-assembly onto DNA to form a gold nanoparticle network with nanogaps. The results of SERS detection of different types of small molecule compounds such as rhodamine G (R6G), pyridine (Py) and p-aminothiophenol (4-ATP) using this substrate showed that this method has high yield and uniform , With good SERS enhancement effect, SERS signal stability and repeatability are greatly improved compared with the conventional isolated gold nanoparticles SERS substrate.