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目的探讨南京地区一般女性人群宫颈细胞、宫颈上皮内瘤变及宫颈癌组织中23种人乳头瘤病毒感染基因型别的分布情况及其临床意义。方法采用基因扩增结合基因芯片技术对1000例一般女性人群宫颈细胞、108例宫颈上皮内瘤变及62例宫颈癌组织标本进行23种HPV基因型别的检测,并对受检者进行相关资料分析。结果 1000例一般女性人群检出HPV感染者106例,总的HPV感染率为10.60%(106/1000);108例宫颈上皮内瘤变患者检出HPV感染者99例,总的HPV感染率为91.67%(99/108);62例宫颈癌患者检出HPV感染者52例,总的HPV感染率为83.87%(52/62)。结论基因扩增结合基因芯片检测技术可应用于宫颈细胞和组织标本,一次可检测23种HPV基因型别,特异性强,敏感性高,对中国女性宫颈HPV感染分子流行病学的调查、宫颈癌和癌前病变的防治及其疫苗的研究具有重要的意义。
Objective To investigate the distribution and clinical significance of 23 human papillomavirus (HPV) genotypes in cervical cancer, cervical intraepithelial neoplasia and cervical cancer in the general female population in Nanjing area. Methods Twenty-three HPV genotypes were detected in 1000 cases of cervical cancer, 108 cases of cervical intraepithelial neoplasia and 62 cases of cervical cancer by gene amplification combined with gene chip technique. The related data analysis. Results 106 cases of HPV infection were detected in 1000 female population, the total HPV infection rate was 10.60% (106/1000). In 108 cases of cervical intraepithelial neoplasia, 99 cases of HPV infection were detected, the total HPV infection rate was 91.67% (99/108). Of 62 cases with cervical cancer, 52 cases were detected with HPV infection, the total HPV infection rate was 83.87% (52/62). Conclusion Gene amplification combined with gene chip detection technology can be applied to cervical cells and tissue samples, a detectable 23 kinds of HPV genotypes, strong specificity and high sensitivity to Chinese women’s cervical HPV infection molecular epidemiological survey, cervical Preventive and precancerous lesions of cancer and vaccine research is of great significance.