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目的:探讨环氧合酶-2(COX-2)和nm23蛋白在胃癌组织中的表达及意义。方法:应用免疫组化方法检测了52胃癌组织及20正常胃黏膜中COX-2和nm23的表达,并在显微镜下计数阳性细胞数,统计比较胃癌组织和正常组织中COX-2和nm23的阳性表达率。结果:COX-2蛋白在胃癌组织和正常胃黏膜组织中阳性表达率分别为67.3%和5.0%,胃癌组织中COX-2蛋白阳性率显著高于正常胃黏膜(P<0.01)。nm23蛋白在胃癌组织和正常胃黏膜组织中阳性表达率分别为30.7%和75.0%,胃癌组织中nm23蛋白阳性率显著低于正常胃黏膜(P<0.01)。COX-2的异常表达与肿瘤细胞分化程度、肿瘤浸润深度、淋巴结转移、TNM分期显著相关(P<0.05)。nm23阳性表达率与肿瘤浸润深度、淋巴结转移、TNM分期显著相关(P<0.05),而与肿瘤细胞分化程度无显著性相关(P>0.05)。结论:COX-2与nm23表达与胃癌的临床病理特征密切相关。COX-2与nm23可作为反映胃癌侵袭转移、判断预后的生物学指标。
Objective: To investigate the expression and significance of cyclooxygenase-2 (COX-2) and nm23 protein in gastric cancer. Methods: The expressions of COX-2 and nm23 in 52 gastric cancer tissues and 20 normal gastric mucosa were detected by immunohistochemistry. The number of positive cells was counted under microscope. The positive rates of COX-2 and nm23 in gastric cancer tissues and normal tissues were statistically compared Expression rate. Results: The positive rate of COX-2 protein in gastric cancer tissue and normal gastric mucosa was 67.3% and 5.0%, respectively. The positive rate of COX-2 protein in gastric cancer tissues was significantly higher than that in normal gastric mucosa (P <0.01). The positive rates of nm23 protein in gastric cancer tissues and normal gastric mucosa tissues were 30.7% and 75.0%, respectively. The positive rates of nm23 protein in gastric cancer tissues were significantly lower than those in normal gastric mucosa (P <0.01). The abnormal expression of COX-2 was significantly correlated with tumor cell differentiation, tumor invasion depth, lymph node metastasis and TNM staging (P <0.05). The positive rate of nm23 was significantly correlated with the depth of tumor invasion, lymph node metastasis and TNM stage (P <0.05), but not with the degree of differentiation of tumor cells (P> 0.05). Conclusion: The expressions of COX-2 and nm23 are closely related to the clinicopathological features of gastric cancer. COX-2 and nm23 can be used as biological indicators to reflect the invasion and metastasis of gastric cancer and prognosis.