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目的:探讨柚苷对脑外伤的防治价值及可能机制。方法:将48只大鼠平均分为甲、乙2组,分别用于生物化学检验和神经行为学观察。将甲、乙组大鼠分别随机平均分为4个小组,每组各6只。A组(对照组),除不进行打击外,其余操作同模型组;B组(模型组),自由落体打击法制作脑外伤大鼠模型;C组(柚苷干预组),造模后给予柚苷(50 mg/kg)治疗7 d;D组(柚苷防治组),造模前、后各7 d均给予柚苷。造模前、后分别对乙组大鼠进行神经行为学评分;对甲组大鼠测量其脑皮层和血清中超氧化物歧化酶和丙二醛的含量。结果:与A组比较,B组大鼠损伤脑组织和血液中丙二醛含量增高,超氧化物歧化酶活性降低;与B组比较,C,D组大鼠损伤脑组织和血液中丙二醛含量降低,超氧化物歧化酶活性增高,以D组变化更显著。与A组比较,B组成模后神经行为学评分较高;与B组比较,C,D组神经行为学评分降低;与C组比较,D组作用更明显,神经行为学评分低。结论:柚苷干预有利于减轻大鼠脑外伤后的神经病理损害,促进功能恢复。柚苷的保护作用可能与缓解脑外伤后的氧化应激有关。
Objective: To explore the prevention and treatment of naringin on brain trauma and its possible mechanism. Methods: A total of 48 rats were divided into A and B groups, respectively, for biochemical test and neurobehavioral observation. A, B rats were randomly divided into four groups randomly, each group of 6. In group A (control group), the rats in model group were divided into control group and model group. Rats in group B (model group) and free-fall attack were used to make model of traumatic brain injury. Group C (naringin intervention group) Naringin (50 mg / kg) was administered for 7 days. In group D (naringin control group), naringin was given before and 7 days after modeling. Before and after modeling, neurobehavioral scores of rats in group B were respectively scored. The content of superoxide dismutase and malondialdehyde in cerebral cortex and serum of rats in group A were measured. Results: Compared with group A, the content of malondialdehyde and the activity of superoxide dismutase in brain tissue and blood of group B were decreased. Compared with group B, the levels of malondialdehyde Aldehyde content decreased, superoxide dismutase activity increased to D group more significant changes. Compared with group A, group B had a higher score of neurological behavior. Compared with group B, neurobehavioral scores of group C and group D were decreased. Compared with group C, group D was more effective and neurobehavioral score was lower. Conclusion: Naringin intervention is beneficial to relieve the neuropathological damage after traumatic brain injury in rats and promote functional recovery. The protective effects of naringin may be related to the alleviation of oxidative stress after traumatic brain injury.