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氨甲酰磷酸合成酶I(CPSI)cDNA全序列基因片段与pSV_2载体的重组及重组质粒pSV_2-CPScf在7402人肝癌细胞、CHO细胞和NIH/3T3细胞中的表达结果表明,pSV_2-CPScf转染的以上3株细胞均可稳定、高效地表达CPSI。通过核Run-off转录活性的检测和Northern杂交证实,表达的CPSI转录产物结构完整。提示CPSI肝组织特异表达主要与转录改变有关。
The recombinant full-length cDNA fragment of carbamyl phosphate synthase I (CPSI) and pSV 2 vector and the expression of recombinant plasmid pSV_2-CPScf in 7402 human hepatoma cells, CHO cells and NIH / 3T3 cells showed that pSV_2-CPScf transfection Of the above three cells can be stable and efficient expression of CPSI. The expression of CPSI transcripts was confirmed to be intact by the detection of nuclear Run-off transcriptional activity and Northern blotting. Tip CPSI liver tissue-specific expression is mainly related to transcriptional changes.