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本文报道以溴化率为基础的差示动力学法,应用于从蓖麻(Ricinus communis L.)叶和水飞蓟[Silybummarianum(L.)Gaertn.]果实中分别提取具有生理活性的黄酮类化合物——芦丁和水飞蓟素(Silybin)的含量测定。其特点为简便、快速、节约、无需特殊设备。芦丁和水飞蓟素分子上的酚基,可使邻位、对位产生溴代反应。在降低介质温度至4～5°时,稳态浓度的溴化速率起到“化学钟”的作用。溴代反应完成后,以甲橙褪色时间T(秒)对浓度C(mg/ml)绘制标准曲线,其线性回归方程式为:T=a+bC(a为截距,b为斜率)。对标准品:Ts=S+bCs,对未知样品:Tu=a+bCu。
In this paper, a differential kinetic method based on the bromination rate was reported and applied to the extraction of physiologically active flavonoids from the fruits of Ricinus communis L. and Silybum marianum (L.) Gaertn. Compound - Rutin and silybin (Silybin) content determination. Its characteristics are simple, fast, economical, no special equipment. Rutin and silymarin on the molecular phenol, can make ortho, para bromine reaction. The steady-state bromination rate plays a role of “chemical clock” when decreasing the medium temperature to 4 ~ 5 °. After the bromination reaction is completed, a standard curve is plotted against the concentration C (mg / ml) of the fade time of the orange T (sec). The linear regression equation is: T = a + bC where a is the intercept and b is the slope. For standard products: Ts = S + bCs, for unknown samples: Tu = a + bCu.