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目的:评估VEGF-C、淋巴管密度(LMVD)与结肠癌临床病理指标及预后的关系。方法:对44例原发结肠癌和14例淋巴结转移癌分别用抗VEGF-C和抗VEGFR-3单抗进行免疫组化染色,检测VEGF-C和VEGFR-3表达,计数LMVD,分析上述指标与临床病理指标和预后的关系以及VEGF-C与LMVD的相关关系。结果:VEGF-C表达率结肠癌为43.2%(19/44),淋巴结转移癌为92.9%(13/14)。LMVD平均为10.14±4.19。VEGF-C表达与肿瘤分化程度(P=0.003)、淋巴结转移(P=0.002)相关。LMVD与淋巴结转移(P=0.001)相关。VEGF-C阳性表达组LMVD为11.34±4.83,高于VEGF-C阴性表达组的9.24±3.48,但VEGF-C与LMVD无相关性(P=0.105)。VEGF-C阳性病人的生存率明显低于VEGF-C阴性病人(P=0.0225),LMVD高密度组病人的生存率明显低于LMVD低密度组病人(P=0.0036)。远处转移(P=0.0004)、淋巴结转移(P=0.021)、LMVD(P=0.0469)可以作为结肠癌独立的预后指标。结论:VEGF-C、LMVD在结肠癌淋巴结转移中起重要作用,VEGF-C和LMVD对于判断结肠癌侵袭性和预后有重要参考价值。LMVD可以作为判断结肠癌预后的独立指标。
Objective: To evaluate the relationship between VEGF-C, lymphatic vessel density (LMVD) and clinicopathological parameters and prognosis of colon cancer. Methods: Immunohistochemical staining of anti-VEGF-C and anti-VEGFR-3 mAb was performed on 44 primary colon carcinomas and 14 metastatic lymph nodes. The expressions of VEGF-C and VEGFR-3 were detected by immunohistochemistry and LMVD were counted. And the relationship between clinicopathological parameters and prognosis and the relationship between VEGF-C and LMVD. Results: The rate of VEGF-C expression was 43.2% (19/44) in colon cancer and 92.9% (13/14) in lymph node metastasis. The average LMVD was 10.14 ± 4.19. The expression of VEGF-C was correlated with tumor differentiation (P = 0.003) and lymph node metastasis (P = 0.002). LMVD was associated with lymph node metastasis (P = 0.001). The LMVD of VEGF-C positive group was 11.34 ± 4.83, which was higher than that of VEGF-C negative group (9.24 ± 3.48), but there was no correlation between VEGF-C and LMVD (P = 0.105). The survival rate of patients with positive VEGF-C was significantly lower than that of patients with negative VEGF-C (P = 0.0225). The survival rate of patients with LMVD high density was significantly lower than that of patients with LMVD low density (P = 0.0036). Distant metastasis (P = 0.0004), lymph node metastasis (P = 0.021) and LMVD (P = 0.0469) could be used as independent prognostic indicators for colon cancer. Conclusion: VEGF-C and LMVD play an important role in lymph node metastasis of colon cancer. VEGF-C and LMVD are important for judging the invasiveness and prognosis of colon cancer. LMVD can be used as an independent indicator of the prognosis of colon cancer.