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建立同时测定白刺果中没食子酸、儿茶素、杨梅素、芦丁、槲皮素、木犀草素、山奈酚和异鼠李素8种黄酮化合物含量的HPLC的方法。采用HYPERSIR C18色谱柱(250 mm×4.6 mm,5μm),柱温35℃,以甲醇-0.1%甲酸水溶液为流动相,进行梯度洗脱,双波长检测(λ1=270 nm,λ2=370 nm),进样量10μL,流速0.8 m L/min。结果:在0~125μg/m L内8种黄酮均有良好的线性关系(R2≥0.999 0),没食子酸、儿茶素、杨梅素、芦丁、槲皮素、木犀草素和山奈酚的平均回收率分别为98.42%,99.19%,90.65%,102.32%,96.51%,98.70%和93.68%,最低检出限依次为0.032,0.052,0.074,0.038,0.059,0.043,0.021和0.036 mg/L。方法精密度RSD≤2.21%(n=6),在此条件下,测得白刺果实中没食子酸、儿茶素、杨梅素、芦丁、槲皮素、木犀草素和山奈酚含量为0.031,0.057,0.064,0.068,0.016和0.038 mg/g。该方法快速简单,重现性好,可为白刺果实质量控制提供定量分析方法。
A method for the simultaneous determination of gallic acid, catechin, myricetin, rutin, quercetin, luteolin, kaempferol and isorhamnetin was developed by HPLC. The gradient elution was carried out on a HYPERSIR C18 column (250 mm × 4.6 mm, 5 μm) with a column temperature of 35 ° C. using a mobile phase of methanol-0.1% formic acid as mobile phase with dual wavelength detection (λ1 = 270 nm, λ2 = 370 nm) , Injection volume 10μL, flow rate 0.8 m L / min. Results: There was a good linear relationship between 8 flavonoids in the range of 0 ~ 125 μg / mL (R2≥0.999 0), gallic acid, catechin, myricetin, rutin, quercetin, luteolin and kaempferol The average recoveries were 98.42%, 99.19%, 90.65%, 102.32%, 96.51%, 98.70% and 93.68%, respectively. The lowest detection limits were 0.032,0.052,0.074,0.038,0.059,0.043,0.021 and 0.036 mg / L . The RSD of the method was 2.21% (n = 6). The content of gallic acid, catechin, myricetin, rutin, quercetin, luteolin and kaempferol was 0.031 , 0.057, 0.064, 0.068, 0.016, and 0.038 mg / g. The method is rapid, simple and reproducible, which can provide a quantitative analysis method for the quality control of Nitraria.