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目的:研究妊娠期糖尿病(GDM)对雌性子代成年以后糖代谢的影响,并初步探讨其机制。方法:造模采用8周龄健康成年雌性SD大鼠,分别与健康雄鼠交配。妊娠当天,GDM组母鼠给予35mg/kg STZ溶液腹腔注射,母鼠对照组腹腔注射缓冲液。两组雌性子代分别记为子代对照组和糖尿病后代组。酶联免疫吸附测定(ELISA)试剂盒检测血清胰岛素、血脂;糖耐量实验、胰岛素耐量实验和胰岛素释放实验检测其胰岛素敏感性;苏木精-伊红染色法(HE)检测胰岛形态学,q PCR检测胰腺microRNA表达。结果:糖尿病后代组大鼠比子代对照组表现轻微的胰岛素抵抗。相对于子代对照组,糖尿病后代组胰岛面积减少,miRNA-130a表达降低。结论:母亲GDM可能是诱发子代糖代谢紊乱的一个高风险因素。
Objective: To study the effect of gestational diabetes mellitus (GDM) on glucose metabolism in adult offspring of adult females and to explore its mechanism. Methods: Eight-week-old healthy adult female Sprague-Dawley rats were mated to healthy male rats. On the day of pregnancy, the female rats in GDM group were given intraperitoneal injection of 35 mg / kg STZ solution, and the control group of the female rats injected intraperitoneally with buffer solution. Two groups of female offspring were recorded as offspring control group and diabetic offspring group. Serum insulin and lipids were detected by enzyme linked immunosorbent assay (ELISA) kit. Insulin sensitivity was measured by glucose tolerance test, insulin tolerance test and insulin release test. Islet morphology was detected by hematoxylin-eosin staining PCR detection of pancreatic microRNA expression. RESULTS: Diabetic offspring rats displayed milder insulin resistance than their offspring controls. Compared with the offspring control group, the islet area of diabetic offspring decreased and the expression of miRNA-130a decreased. Conclusion: Mother GDM may be a high risk factor for inducing glucose metabolism disorder in offsprings.