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目的：研究华蟾素对体外培养的人胃癌、结肠癌、肝癌细胞的杀伤作用。方法：ＭＴＴ 法检测细胞增殖抑制率；光镜观察细胞形态改变；琼脂糖凝胶电泳观察ＤＮＡ 断裂。结果：三种肿瘤细胞在华蟾素（２ ｍｇ／ ｍｌ）孵育４８ 小时后其生长抑制率为１６－６ ％ ～２３－３ ％ ，细胞形态由多边形变成圆形，染色质疏松，细胞核溶解，ＤＮＡ电泳显示连续膜状条带。结论：华蟾素直接损伤肿瘤细胞ＤＮＡ，并由此导致细胞坏死
Objective: To study the killing effect of Huachansu on human gastric cancer, colon cancer and liver cancer cells cultured in vitro. Methods: MTT assay was used to detect the inhibition of cell proliferation; light microscopy was used to observe the changes of cell morphology; DNA fragmentation was observed by agarose gel electrophoresis. RESULTS: The growth inhibition rates of the three tumor cells at the time of 48 h after inoculation of Cinobufotalin (2 mg/ml) were 16-6 % to 23-3 %. The cell morphology changed from polygon to round, chromatin was loose, and the nucleus was dissolved. DNA electrophoresis shows a continuous membrane band. Conclusion: Cinobufacini directly damages DNA of tumor cells and leads to cell necrosis