论文部分内容阅读
目的:建立酒石酸茚喹诺啉脂质体中药物含量及包封率测定方法。方法:采用逆向蒸发法制备脂质体,葡聚糖凝胶柱层析法分离脂质体和游离药物,以磷酸盐缓冲液为洗脱介质。采用反相高效液相色谱法测定脂质体中酒石酸茚喹诺啉的含量。结果:酒石酸茚喹诺啉含量测定方法的回收率为99.5%,在5~200μg.mL-1范围内线性关系良好(r=0.9998);柱层析法分离游离药物的柱回收率为101.0%,加样回收率为97.9%。样品的平均包封率为40.3%。结论:酒石酸茚喹诺啉脂质体中药物含量及包封率测定方法准确、灵敏,可用于测定酒石酸茚喹诺啉脂质体的包封率。
OBJECTIVE: To establish a method for the determination of entrapment efficiency and entrapment factor in quinolinic acid liposomes. METHODS: Liposomes were prepared by reverse-phase evaporation. Liposomes and free drugs were separated by Sephadex LH-20 column chromatography. Phosphate buffer was used as elution medium. Inverse phase high performance liquid chromatography was used to determine the content of indene quinolinic acid in liposomes. Results: The recovery of quinolinic acid content was 99.5%, and the linearity was good (r = 0.9998) in the range of 5 ~ 200μg.mL-1. The column recovery of free drug was 101.0% , Sample recovery rate of 97.9%. The average encapsulation efficiency of the sample was 40.3%. Conclusion: The method for determination of entrapment rate and entrapment efficiency of indene quinolinyl tartrate liposomes is accurate and sensitive. It can be used to determine the entrapment efficiency of quinolinic acid liposomes.