消灭脊灰是预防医学的重要课题,1991年时我国仍是世界上脊灰流行区之一,其主要病原为Ⅰ型脊髓灰质炎病毒(PV1),为弄清PV在我国的流行情况,我们组织全国主要省区防疫站协作自选进行“我国PV1野毒株的分子流行病学研究”课题。地方同志负责脊灰病人粪便样品的采集、病毒的分离和部分病毒血清型检定,本实验室负责从各地的PV分离株中用RT—PCR和打点杂交方法区别疫苗相关株或野毒株,选PV1 Polio eradication is an important issue in preventive medicine. In 1991, China was still one of the poliovirus endemic areas in the world. Its main pathogen is poliovirus type 1 (PV1). To understand the prevalence of PV in China, we Organize major provinces and autonomous regions epidemic prevention station collaboratively choose “China’s PV1 wild strains of molecular epidemiology research” subject. Local comrades are responsible for the collection of excrement samples of polio patients, the isolation of viruses and the detection of some virus serotypes. The laboratory is responsible for distinguishing vaccine-associated strains or wild-type strains from PV isolates from different places by RT-PCR and dot blot hybridization PV1