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目的 :建立腺样囊性癌细胞与大鼠施万细胞共培养模型,探讨细胞间相互作用时BDNF、TrkB和E-cadherin的表达变化情况。方法:采用Transwell系统建立腺样囊性癌细胞与大鼠施万细胞共培养模型,共培养96 h后,观察肿瘤细胞的形态学变化,以ELISA法检测各组培养液中BDNF含量,实时定量PCR(RT-PCR)、Western免疫印迹检测肿瘤细胞中BDNF、TrkB及E-cadherin的表达情况。采用SPSS17.0软件包对实验结果进行统计学处理。结果:与施万细胞共培养的腺样囊性癌细胞发生长梭形及多角形改变,E-cadherin表达下调,TrkB表达上调,BDNF表达未见明显变化。与腺样囊性癌细胞系共培养的培养液中,施万细胞表达BDNF的量比单独培养的施万细胞显著增高;而对照组黏液表皮样癌细胞与施万细胞共培养时未发生类似改变。结论:BDNF/TrkB信号通路在唾液腺腺样囊性癌嗜神经侵袭过程中发挥重要作用,但其具体分子机制有待进一步研究。
OBJECTIVE: To establish a co-culture model of adenoid cystic carcinoma cells and rat Schwann cells in order to explore the changes of the expression of BDNF, TrkB and E-cadherin in cell-cell interactions. Methods: The co-culture model of adenoid cystic carcinoma cells and rat Schwann cells was established by Transwell system. After cultured for 96 h, the morphological changes of tumor cells were observed. The content of BDNF in each group was detected by ELISA, The expression of BDNF, TrkB and E-cadherin in tumor cells were detected by RT-PCR and Western immunoblotting. SPSS17.0 software package for the experimental results for statistical analysis. RESULTS: The adenoid cystic carcinoma cells co-cultured with Schwann cells exhibited long-fusiform and polygonal changes. The expression of E-cadherin was down-regulated and TrkB was up-regulated. The expression of BDNF did not change significantly. In the culture medium co-cultured with the adenoid cystic carcinoma cell line, the amount of BDNF expressed by Schwann cells was significantly higher than that of the Schwann cells cultured alone; while in the control group, mucoepidermoid carcinoma cells were not co-cultured with Schwann cells change. CONCLUSION: The BDNF / TrkB signaling pathway plays an important role in the process of esophageal adenoid cystic carcinoma (NSCLC) invasion. However, its specific molecular mechanism remains to be further studied.