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目的探讨Bax、Bcl-2在大鼠微波辐射模型脾脏组织中的表达及意义,为揭示微波辐射致免疫损伤的相关机制提供实验依据。方法以电磁脉冲(electromagnetic pulse,EMP)、S波段高功率微波(S-band high power microwave,S-HPM)和X波段高功率微波(X-band high power microwave,X-HPM)三种不同波段的微波为辐射源,建立大鼠微波辐射损伤模型,采用免疫组织化学(immunohistochemistry,IHC)技术结合半定量技术,检测Bax、Bcl-2蛋白在模型脾脏组织内表达的变化。所得数据采用SPSS 13.0软件进行一元方差分析。结果 3个实验组脾脏Bax蛋白表达在各时间点均轻微上调,Bcl-2蛋白表达于照后6 h、3 d和7 d均轻微上调,于照后14 d和28 d均轻微下调,经统计学分析,实验组与对照组比较差异均无统计学意义。结论微波辐射大鼠,虽未导致其脾脏内Bax、Bcl-2蛋白表达发生显著改变,但不能完全否认二者在微波辐射致大鼠脾脏损伤中的作用。
Objective To investigate the expression and significance of Bax and Bcl-2 in the rat model of microwave radiation and to provide experimental evidence for revealing the mechanism of immune damage induced by microwave radiation. Methods Three different wavebands of electromagnetic pulse (EMP), S-band high power microwave (S-HPM) and X-band high power microwave (X-HPM) The microwave radiation was used as the radiation source to establish the rat model of radiation injury. The expression of Bax and Bcl-2 protein in the model spleen tissue was detected by immunohistochemistry (IHC) and semi-quantitative techniques. The data obtained using SPSS 13.0 software for one-way analysis of variance. Results The expression of Bax protein in spleen of three experimental groups was slightly up-regulated at each time point. The expression of Bcl-2 protein was slightly up-regulated at 6 h, 3 d and 7 d after irradiation and slightly down-regulated at 14 and 28 d Statistical analysis, there was no significant difference between the experimental group and the control group. Conclusion The microwave irradiation did not cause a significant change in the expression of Bax and Bcl-2 in the spleen of rats, but it can not completely deny the effect of microwave irradiation on the spleen injury in rats.