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目的探讨泽泻提取物中提取的萜类衍生物单体V-54对小RNA病毒感染增殖的抑制效应及其生物学机理。方法采用KMB17细胞,分别感染HAV-H、PV-Ⅰ和Cox-B2病毒,经V-54处理后检测病毒滴度,分析PV-Ⅰ和Cox-B2病毒增殖抑制的动力学及V-54对KMB17细胞凋亡的影响。结果V-54可抑制3种病毒在KMB17细胞上增殖,PV-Ⅰ和Cox-B2病毒感染滴度比未处理组明显降低,V-54对两种病毒的抑制作用呈剂量依赖性。作用24h内,V-54可使KMB17细胞凋亡率明显增加,随后逐渐恢复正常。结论V-54可抑制特定的小RNA病毒的感染增殖,其机制可能为V-54分子结合到细胞表面的相关受体,导致病毒感染效率降低。
OBJECTIVE To investigate the inhibitory effect of terpenoid monomer V-54 extracted from Alisma orientalis extract on the proliferation of murine parvovirus infection and its biological mechanism. Methods KMB17 cells were used to infect HAV-H, PV-I and Cox-B2 viruses respectively. The virus titer was detected after treatment with V-54. The kinetics of inhibition of PV-I and Cox-B2 virus proliferation and V-54 KMB17 cell apoptosis. Results V-54 inhibited the proliferation of KMB17 cells. The titer of PV-I and Cox-B2 virus was significantly lower than that of untreated cells. V-54 inhibited the proliferation of KMB17 cells in a dose-dependent manner. Within 24 hours, V-54 could significantly increase the apoptosis rate of KMB17 cells and then gradually returned to normal. Conclusions V-54 inhibits the proliferation of specific picornavirus infection. The mechanism may be that V-54 binds to related receptors on cell surface, leading to the decrease of virus infection efficiency.