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目的研究低氧培养大鼠心肌细胞Na+通道基因SCN5A mRNA表达的变化,并探讨其与丙二醛(M alond ialhyde,MDA)含量及超氧化物歧化酶(superoxide d ismutase,SOD)活性的关系。方法培养大鼠心肌细胞,检测各组培养液氧分压。用RT-PCR检测SCN5A mRNA表达,检测培养液MDA含量和细胞SOD活性。结果与对照组相比:SCN5A mRNA相对表达量低氧1 h和6 h组显著增高(P<0.001)、低氧12 h和24 h组显著降低(P<0.001);MDA含量显著增高而SOD活性显著降低。结论心肌细胞Na+通道基因SCN5A在低氧1 h和6 h mRNA表达上调,而在低氧12 h和24 h mRNA表达下调,且与脂质过氧化和SOD相关。
Objective To investigate the changes of Na + channel gene SCN5A mRNA expression in rat cardiac myocytes under hypoxia and investigate its relationship with the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD). Methods Cardiac myocytes were cultured and the oxygen partial pressure in each group was detected. The expression of SCN5A mRNA was detected by RT-PCR. The content of MDA and the activity of SOD in the culture medium were detected. Results Compared with the control group, the relative expression of SCN5A mRNA was significantly increased in hypoxia for 1 h and 6 h (P <0.001), and decreased significantly in hypoxia for 12 h and 24 h (P <0.001) Significantly reduced activity. Conclusions The expression of Na + channel gene SCN5A in cardiomyocytes was up-regulated at 1 h and 6 h of hypoxia, down-regulated at 12 h and 24 h of hypoxia, and correlated with lipid peroxidation and SOD.