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目的探讨苯并[a]芘(BaP)与铅对小鼠学习记忆能力影响之间的协同效应。方法将80只昆明小鼠随机分为10组:空白对照组,溶剂对照组(等量花生油),低、高铅组(5.4、54.0 mg/L),低、高Ba P组(0.5、5.0mg/kg),低铅+低Ba P组,低铅+高Ba P组,高铅+低Ba P组,高铅+高Ba P组;采用M orris水迷宫评定小鼠空间学习记忆能力,单细胞凝胶电泳检测海马神经元DNA损伤。结果与对照组比较,低、高铅组,低、高Ba P组,低铅+低Ba P组,低铅+高Ba P组,高铅+低Ba P组,高铅+高Ba P组小鼠逃避潜伏期[分别为(25.66±2.93)、(28.43±3.24),(23.56±2.69)、(33.54±3.82),(28.52±3.25),(43.57±4.97),(40.26±4.59),(54.54±6.22)s]明显延长,而跨越平台次数及目标象限花费时间明显减少,差异均有统计学意义(P<0.001);与对照组比较,低、高铅组,低、高Ba P组,低铅+低Ba P组,低铅+高Ba P组,高铅+低Ba P组,高铅+高Ba P组小鼠尾部DNA百分比(分别为0.09%、0.15%,0.12%、0.21%,0.17%,0.31%,0.31%,0.51%)明显增加(F=9.823、5.226,P<0.05)。结论苯并[a]芘与铅联合对小鼠空间学习记忆损伤具有协同效应,其机制可能与氧化应激损伤有关。
Objective To investigate the synergistic effect of benzo [a] pyrene (BaP) and lead on learning and memory in mice. Methods Eighty Kunming mice were randomly divided into 10 groups: blank control group, solvent control group (equivalent peanut oil), low and high lead group (5.4, 54.0 mg / L), low and high Ba P group low Pb + low Ba P group, low Pb + high Ba P group, high Pb + low Ba P group, high Pb + high Ba P group. The spatial learning and memory ability of mice was evaluated by Mris water maze, Detection of DNA damage in hippocampal neurons by single cell gel electrophoresis. Results Compared with the control group, low and high lead group, low and high Ba P group, low lead + low Ba P group, low lead + high Ba P group, high lead + low Ba P group, high lead + high Ba P group The escape latency of mice [(25.66 ± 2.93), (28.43 ± 3.24), (23.56 ± 2.69), (33.54 ± 3.82), (28.52 ± 3.25), (43.57 ± 4.97), (40.26 ± 4.59), 54.54 ± 6.22) s] significantly prolonged, while the time spent crossing the platform and the target quadrant decreased significantly (P <0.001). Compared with the control group, low and high lead group, low and high BaP group (P <0.05), the percentage of tail DNA in low Pb + low Ba P group, low Pb + high Ba P group, high Pb + low Ba P group and high Pb + Ba P group were 0.09%, 0.15%, 0.12% %, 0.17%, 0.31%, 0.31%, 0.51%) (F = 9.823,5.226, P <0.05). Conclusion The combination of benzo [a] pyrene and lead has a synergistic effect on spatial learning and memory impairment in mice. The mechanism may be related to oxidative stress injury.