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应用ＣＴＬＬ２细胞短期培养３Ｈ－ＴｄＲ掺入法和Ｌ９２９细胞结晶紫染色法，观察ＮＩＨ小鼠接种Ｓ１８０实体瘤细胞前后，分别注射ＨＰ－ＴＦ０．５ｍｌ／鼠连续２０天，其脾细胞ＩＬ－２诱生水平和外周血ＴＮＦ活性的变化。结果显示，预防组和治疗组小鼠脾细胞ＩＬ－２诱生水平（ｃｐｍ值）分别为２１２８．７２±１６４．４２、３２９５．９６±２３０．７１，较荷瘤对照组（１２６０．４３±１４５．５７）明显提高（Ｐ＜０．００１）；而外周血ＴＮＦ活性（％）分别为４５．００±５．１８．５２．２９±５．２１，与荷瘤对照组（２５．８３±５．５２）比较有非常显著性差异（ｐ＜０．００１）。两组的抑瘤率分别为６８．８８％和６５．８２％。提示ＨＰ－ＴＦ能显著提高荷瘤小鼠脾细胞ＩＬ－２诱生水平和外周血ＴＮＦ活性，且能明显抑制Ｓ１８０实体瘤细胞在小鼠体内的生长。木研究结果有助于对ＨＰ－ＴＦ提高荷瘤机体细胞免疫功能的机理的探讨，并为临床应用ＨＰ－ＴＦ治疗肿瘤患者提供了实验依据。
3H-TdR incorporation of CTLL2 cells and crystal violet staining of L929 cells were used to observe the effect of IL-2 induced by splenocytes induced by HP-TF 0.5 ml / mouse for 20 days in NIH mice before and after inoculation of S180 solid tumor cells Changes in plasma levels and TNF activity in peripheral blood. The results showed that the IL-2 induced levels (cpm values) of spleen cells in preventive group and treatment group were 2128.72 ± 164.42 and 3295.96 ± 230.71, respectively, which were significantly higher than those in the control group (1260.43 ± 145.57) (P <0.001), while the activity of TNF in peripheral blood (%) was 45.00 ± 5.18.52.29 ± 5.21, which was significantly higher than that in tumor-bearing control group (25.83 ± 5.52) compared with a very significant difference (p <0.001). The inhibition rates of the two groups were 68.88% and 65.82% respectively. Tip HP-TF can significantly increase the level of IL-2 induced by spleen cells in tumor-bearing mice and TNF activity in peripheral blood, and can significantly inhibit the growth of S180 solid tumor cells in mice. The results of the study of wood will help to explore the mechanism of HP-TF in enhancing the cellular immune function of tumor-bearing cells and provide experimental evidence for the clinical application of HP-TF in the treatment of cancer patients.