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目的 研究大黄对烫伤后大鼠肠黏膜上皮细胞线粒体呼吸链的影响。 方法 采用大鼠背部烫伤模型 ,随机分为大黄治疗组、烫伤组和正常对照组。分离小肠黏膜上皮细胞内线粒体 ,监测呼吸控制率 (RCR)、琥珀酸脱氢酶和烟酰胺腺嘌呤二核苷酸 (NADH)脱氢酶活力和细胞色素C的含量。 结果 烫伤后各时相点NADH和琥珀酸氧化呼吸链的RCR显著低于对照组 ,细胞色素C亦有同样改变 ,而烫伤对琥珀酸脱氢酶和NADH脱氢酶活力无显著影响。大黄能提高上述两条呼吸链的RCR水平 ,降低线粒体内膜细胞色素C的丢失 (与烫伤组比较 ,P <0 .0 5 )。 结论 烫伤能导致大鼠肠黏膜上皮细胞线粒体呼吸链明显受损 ,而大黄能明显改善线粒体的呼吸功能
Objective To study the effect of rhubarb on the mitochondrial respiratory chain of intestinal epithelial cells in rats after scald. Methods Rat back scald model was randomly divided into rhubarb treatment group, scald group and normal control group. Mitochondria were isolated from intestinal mucosal epithelial cells and monitored for respiratory control rate (RCR), succinate dehydrogenase, and nicotinamide adenine dinucleotide (NADH) dehydrogenase activity and cytochrome C content. Results After burns, the RCR of NADH and succinic acid oxidized respiratory chain at each time point was significantly lower than that of the control group. Cytochrome C also changed similarly, and scald had no significant effect on the activity of succinate dehydrogenase and NADH dehydrogenase. Rhubarb can increase the RCR levels of the two respiratory chains and reduce the loss of mitochondrial inner membrane cytochrome C (compared with the scald group, P < 0.05). Conclusion Scald can lead to significantly impaired mitochondrial respiratory chain in rat intestinal mucosal epithelial cells, while rhubarb can significantly improve mitochondrial respiratory function.