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应用CM和SP琼脂糖凝胶 (Sepharose)离子交换层析法从‘新水’梨花柱可溶性蛋白质中分离纯化出具有RNase活性的S糖蛋白 ,将其加入花粉培养基 3～ 12h ,新水花粉 (自交不亲和 )的萌发率及花粉管生长受到强烈抑制 ,当浓度为 1.2 5μg·μL- 1时 ,分别为对照的 6 5%和 38% ,低浓度 (0 .4 μg·μL- 1)抑制作用不明显 ;与新水杂交不亲和的‘幸水’花粉也出现同样的结果 ;而与其杂交亲和的‘长十郎’花粉萌发率不受影响 ,仅花粉管生长受到轻微的抑制 ,其长度为对照的 79%。不含S糖蛋白的花柱可溶性蛋白质对自花及异花的花粉萌发及花粉管生长均无影响。
The RNase-active S-glycoprotein was isolated and purified from the soluble protein of ’Xinshui’ pear by CM and SP Sepharose ion exchange chromatography. The S glycoprotein was added to the pollen medium for 3 ~ 12 h, (Self-incompatible) germination rate and pollen tube growth were strongly inhibited, when the concentration of 1.2 5μg · μL-1, respectively, 65% and 38% of the control, low concentrations (0.4 μg · μL- 1) the inhibition was not obvious; the same result was also found with the ’Shuihui’ pollen incompatible with Xinshui; however, the pollen germination rate of the hybrid progeny ’Changjuro’ was unaffected and only the pollen tube grew slightly Inhibition, the length of the control 79%. Stylar soluble protein without S glycoprotein had no effect on pollen germination and pollen tube growth.