The development of high-performance glucose sensors is an urgent need,especially for diabetes mellitus diagnosis.However,the glucose monitoring is conventionally operated in an invasive finger-prick man-ner and their noninvasive alternatives largely suffered from the relatively poor sensitivity,selectivity,and stability,resulted from the lack of robust and efficient catalysts.In this paper,we design a concave shaped nitrogen-doped carbon framework embellished with single Co site catalyst(Co SSC)by selectively con-trolling the etching rate on different facet of carbon substrate,which is beneficial to the diffusion and contact of analyte.The Co SSC prompts a significant improvement in the sensitivity of the solution-gated graphene transistor(SGGT)devices,with three orders of magnitude better than those of SGGT devices without catalysts.Our findings expand the field of single site catalyst in the application of biosen-sors,diabetes diagnostics and personalized health-care monitoring.