目的：分析我院２ａ来铜绿假单胞菌医院感染分布及其对不同类型抗生素的耐药情况。方法：对我院各病房送检的痰、尿、分泌物等标本进行细菌培养，并采用肉汤稀释法对头孢菌素类、氨基糖甙类等１１种抗生素进行药物最低抑菌浓度分析。结果：痰标本中铜绿假单胞菌分离率为１１１％，分泌物中为１５４％；菌株的１９５％分布在重症监护病房，１５５％分布在西医甲级病房；抗生素耐药率分别为头孢唑啉９８％、头孢哌酮２２％、头孢他啶１２４％、环丙沙星２０％、庆大霉素３２１％等。结论：铜绿假单胞菌仍是我院细菌感染的主要致病菌之一，主要发生在免疫力低下的老年危重患者中，且对多种不同类型的抗生素耐药，临床应加强合理应用抗生素以减少耐药菌株的产生 Objective: To analyze the distribution of Nosocomial P. aeruginosa nosocomial infection in hospital 2a and its resistance to different types of antibiotics. Methods: Sputum, urine and secretions were collected from various wards of our hospital for bacterial culture. The minimum inhibitory concentrations (MICs) of 11 antibiotics such as cephalosporins and aminoglycosides were determined by broth dilution method. Results: The rates of Pseudomonas aeruginosa isolated from sputum were 111% and 154% respectively. 195% of strains were distributed in intensive care unit and 155% in western medicine ward. The rate of antibiotic resistance was 98% for cefazolin, 22% for cefoperazone, 12.4% for ceftazidime, 20% for ciprofloxacin and 32.1% for gentamicin. Conclusion: Pseudomonas aeruginosa is still one of the main pathogens of bacterial infection in our hospital. It mainly occurs in elderly critically ill patients with low immunity, and is resistant to many different types of antibiotics. Clinical application of antibiotics should be strengthened To reduce the production of resistant strains