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目的:观察三甲散对实验性肝纤维化大鼠转化生长因子(TGF-β)、肝再生增强因子(ALR)的影响,从分子生物学角度探讨其作用机制。方法:SD大鼠100只,50%CCl4植物油溶液ip造模后分为模型组、马洛替酯组(0.1 g·kg-1)及三甲散低、中、高(0.45,0.9,1.8 g·kg-1)剂量组,并设空白组作为对照。给药8周,测定各组大鼠的转氨酶、羟脯氨酸(Hyp)及血清透明质酸(HA)、层黏蛋白(LN)、三型前胶原(PⅢNP)、四型胶原(PⅣP),肝组织TGF-β,ALR的含量;肝组织做病理形态学检查。结果:模型组大鼠转氨酶升高,血清HA,LN,PⅢNP,PⅣP升高,肝组织TGF-β,ALR急剧升高,与空白对照组比较,差异非常显著(P<0.01),病理显示模型组大鼠汇管区及小叶间大量纤维增生。三甲散各剂量组均可降低转氨酶及血清HA,LN,PⅢNP,PⅣP,肝组织TGF-β,ALR含量下降,与模型组比较,差异显著(P<0.05)。结论:三甲散可降低肝纤维化大鼠肝组织中TGF-β,ALR的含量,抑制纤维结缔组织的增生。
Objective: To observe the effects of “Sanjian San” on transforming growth factor (TGF-β) and augmenter of liver regeneration (ALR) in rats with experimental hepatic fibrosis and to explore its mechanism from the perspective of molecular biology. Methods: One hundred SD rats and 50% CCl4 vegetable oil were divided into model group, model group, Mallotide group (0.1 g · kg -1) and SJA low, medium and high (0.45, 0.9 and 1.8 g Kg-1) dose group, and set the blank group as a control. After 8 weeks of administration, the levels of ALT, Hyp, HA, LN, PⅢNP, PⅣP, , Liver tissue TGF-β, ALR content; liver tissue pathological examination. Results: Compared with the blank control group, the levels of ALT, serum HA, LN, PⅢNP and PⅣP in the model group were significantly increased and the levels of TGF-β and ALR in the liver tissue were significantly increased (P <0.01) Rats in the portal area and a large number of fibrous lobular hyperplasia. All the three groups could decrease the levels of HA, LN, PⅢNP, PⅣP, TGF-β and ALR in the liver tissue of the rats in each dose group. The difference was statistically significant (P <0.05). Conclusion: Sanjian Powder can reduce the content of TGF-β, ALR in liver tissue of rats with hepatic fibrosis and inhibit the proliferation of fibrous connective tissue.