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目的:研究血管紧张素转化酶(ACE)基因ID多态性和血管紧张素Ⅱ1型受体(AT1R)基因A1166C多态性与日本高知地区非酒精性脂肪性肝炎(NASH)易感性的相关性,从基因学角度探讨NASH的发生发展机制,为NASH的预防、诊断和治疗提供一定的理论依据。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-PFLP)方法对日本高知地区104例NASH患者和150例正常人ACE-ID基因和AT1R-A1166C基因多态性进行分析。结果:NASH病例组D等位基因频率显著高于正常对照组(P<0.01);NASH病例组的DD基因型频率显著高于正常对照组(P<0.01);NASH病例组A等位基因频率与正常对照组相比差异无显著(P(0.05)。NASH病例组AA基因型频率与正常对照组相比差异无显著(P(0.05)。结论:ACE-ID基因位于内含子16上D等位基因与DD基因型与NASH的发生发展显著相关,可能是促进NASH发生的原因之一;AT1R-A1166C基因多态性与NASH的发生发展尚未显示相关性。
AIM: To investigate the association between angiotensin converting enzyme (ACE) gene ID polymorphism and angiotensin Ⅱ type 1 receptor (AT1R) gene A1166C polymorphism and susceptibility to non-alcoholic steatohepatitis (NASH) in Kochi, Japan , To explore the mechanism of occurrence and development of NASH from the perspective of genetics and to provide a theoretical basis for the prevention, diagnosis and treatment of NASH. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-PFLP) was used to analyze the polymorphism of ACE-ID and AT1R-A1166C genes in 104 NASH patients and 150 normal controls in Kochi Prefecture, Japan. Results: The frequency of D allele in NASH cases was significantly higher than that in normal controls (P <0.01). The frequency of DD genotype in NASH cases was significantly higher than that in normal controls (P <0.01) There was no significant difference between the two groups (P (0.05)). There was no significant difference in AA genotype frequencies between the NASH group and the normal control group (P <0.05) .Conclusion: ACE-ID gene is located on intron 16 Alleles and DD genotypes were significantly associated with the occurrence and development of NASH, which may be one of the reasons to promote the occurrence of NASH. There is no correlation between AT1R-A1166C gene polymorphism and the occurrence and development of NASH.