目的：探讨抑癌基因ｐ５３和癌基因ｍｄｍ２在肺腺癌细胞系ＧＬＣ８２中的相互关系和作用．方法：采用脂质体Ｌｉｐｏｆｅｔｉｎ介导的ＤＮＡ转染法，用含有野生型ｐ５３基因和ｍｄｍ２基因的ＰＤＯＲｎｅｏ逆转录病毒载体分别或共转染ＧＬＣ－８２细胞．结果：转染野生型ｐ５３基因可阻止ＧＬＣ８２细胞生长；抑制ＧＬＣ８２细胞ＤＮＡ合成；软琼脂培养集落形成率减少及裸小鼠成瘤性减慢，而ｍｄｍ２基因的转染可拮抗野生型ｐ５３的功能．结论：将ｍｄｍ２表达载体转染含有野生型ｐ５３基因的ＧＬＣ８２细胞后，ｍｄｍ２可部分地解除野生型ｐ５３对ＧＬＣ８２细胞生长的抑制作用． Objective: To investigate the relationship between tumor suppressor gene p53 and oncogene mdm2 in lung adenocarcinoma cell line GLC82. METHODS: Lipofetin-mediated DNA transfection was used to transfect GLC-82 cells with PDORneo retroviral vectors containing wild-type p53 and mdm2 genes. RESULTS: Transfection of wild-type p53 gene prevented the growth of GLC-82 cells; inhibited DNA synthesis in GLC-82 cells; soft agar culture reduced the rate of colony formation and tumorigenesis in nude mice; transfection of mdm2 gene could antagonize the wild P53 function. Conclusion: After mdm2 expression vector was transfected into GLC82 cells containing wild-type p53 gene, mdm2 could partially relieve the inhibitory effect of wild-type p53 on the growth of GLC82 cells.