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采用圆二色谱（ＣＤ）方法对镧离子（Ｌａ３＋）与转移核糖核酸（ｔＲＮＡ）的相互作用进行了研究．结果显示Ｌａ３＋可使酵母ｔＲＮＡ的ＣＤ谱峰向短波方向偏移３ｎｍ，波峰位于２５４ｎｍ，峰值减少１０％，说明结合在ｔＲＮＡ分子上的Ｌａ３＋引起ｔＲＮＡ分子构象的变化．Ｌａ３＋对氨酰化ｔＲＮＡ合成酶（ａａＲＳ）或ｔＲＮＡ－ａａＲＳ复合物的ＣＤ光谱基本上无影响，说明Ｌａ３＋对氨酰化反应活性的影响，可能是通过改变ｔＲＮＡ分子构象所致
The interaction of lanthanum ion (La3 +) with the transcribed ribonucleic acid (tRNA) was investigated by circular dichroism (CD) method. The results showed that La3 + could shift the CD peak of yeast tRNA toward the short wave direction by 3nm and the peak at 254nm with a peak decrease of 10%, indicating that the conformation of tRNA changes due to La3 + binding to tRNA. The CD spectra of La3 + for the aminoacylated tRNA synthetase (aaRS) or tRNA-aaRS complex showed no significant effect, indicating that the effect of La3 + on the aminoacylation activity may be caused by changing the conformation of the tRNA molecule