论文部分内容阅读
目的探讨生物发光显像技术用于移植胰岛监测的优势和可行性。方法成年雄性C57BL/6小鼠腹腔注射链佐星,制成糖尿病模型。取C57BL/6小鼠和Bclb/c小鼠胰腺,消化、分离、纯化,获得胰岛,再将荧光素酶基因转入胰岛。将糖尿病模型小鼠分为同系移植组(n=20)和同种移植组(n=7)。同系移植组糖尿病模型小鼠移植不同数量(分别为10、50、100和200个,每个数量移植5只小鼠)的C57BL/6小鼠胰岛,同种移植组糖尿病模型小鼠移植Bclb/c小鼠胰岛,胰岛均移植至左后腿上方皮下脂肪内。在设计时间点对受者进行生物发光扫描成像,观察光密度强弱及变化规律,并监测同种移植组受者的随机血糖变化。结果移植后第6天,扫描成像可见移植区光密度随移植胰岛数量增多而增高,光密度与植入胰岛数量呈正相关。同种移植组受者的随机血糖在移植后2 d内迅速下降至正常水平,平均于11 d后再度逐渐升高至糖尿病水平,扫描成像显示移植区光密度在移植后6～7 d达到峰值,随后迅速下降;光密度开始下降时间为移植后(6.14±0.90)d,而血糖升高时间发生在移植后(10.00±0.82)d,前者改变时间明显早于后者(P<0.05)。结论胰岛生物发光显像技术可及时、直观、准确的反映移植胰岛在机体内的存活状况,其成像改变早于血糖变化。
Objective To investigate the advantages and feasibility of bioluminescence imaging in the monitoring of islet transplantation. Methods Adult male C57BL / 6 mice were injected intraperitoneally with streptozocin to make diabetes model. C57BL / 6 mice and Bclb / c mouse pancreas were taken, digested, separated and purified to obtain islets, and the luciferase gene was transferred to islets. Diabetic model mice were divided into three groups: the homograft group (n = 20) and the allograft group (n = 7). The C57BL / 6 mice were transplanted with different numbers of diabetic mice (10, 50, 100 and 200, respectively, 5 mice in each number) in the same line transplantation group. Allograft diabetic mice were transplanted with Bclb / c mouse islets, islets are transplanted to the left hind legs above the subcutaneous fat. The recipients were scanned with bioluminescence scanning at design time points to observe the intensity and variation of optical density and to monitor random blood glucose changes in recipients of allograft recipients. Results On the 6th day after transplantation, the optical density of the transplanted area increased with the number of transplanted islets on the sixth day. The optical density was positively correlated with the number of transplanted islets. In the allograft recipients, the random blood glucose rapidly decreased to normal level within 2 days after transplantation, and then gradually increased to the level of diabetes again after 11 days. Scanning imaging showed that the optical density of the transplantation area peaked at 6-7 days after transplantation , Then decreased rapidly. The optical density began to decline after transplantation (6.14 ± 0.90) d, and the time of blood glucose rise occurred after transplantation (10.00 ± 0.82) d, the former changed significantly earlier than the latter (P <0.05). Conclusion The islet bioluminescence imaging technique can reflect the survival status of transplanted islets in vivo promptly, intuitively and accurately, and its imaging changes are earlier than the change of blood glucose.