论文部分内容阅读
以黑美人粗肋草带腋芽的茎段为外植体,研究不同灭菌方法、不同接种方法以及在培养基中添加不同浓度的植物生长调节剂对黑美人粗肋草离体快繁的影响。结果表明:(1)采用75%酒精擦拭外植体和0.1%HgCl2灭菌处理20 min较适合黑美人粗肋草的离体快繁;(2)采用芽眼朝上水平放置的接种方式较适合不定芽的诱导;(3)不定芽诱导最佳培养基为MS+2.0 mg/L 6-BA+0.1 mg/L NAA,不定芽诱导率达94.10%;(4)通过继代增殖培养筛选出较适合的培养基为MS+4.0 mg/L 6-BA+0.05 mg/L NAA,增殖倍数为6.30倍,最适继代代数为5～6代;(5)幼苗转入1/2MS+0.2～0.3 mg/L NAA的培养基中生根效果较好,生根率达到100%;(6)移栽至泥炭或者菜园土的基质中并保温保湿,成活率较高,分别达到92.30%和88.00%。
In order to study the effects of different sterilization methods, different inoculation methods and different concentrations of plant growth regulators on the ex vivo fast propagation of Coati ribestris . The results showed that: (1) In vitro sterilization with 75% alcohol wipe explants and 0.1% HgCl2 sterilization for 20 min was more suitable for the in vitro propagation of the black-haired Rugosa. (2) The inoculation method (3) Induction of adventitious buds was the best culture medium for MS + 2.0 mg / L 6-BA + 0.1 mg / L NAA, the adventitious bud induction rate was 94.10%; (4) The suitable culture medium was MS + 4.0 mg / L 6-BA + 0.05 mg / L NAA, the multiplication ratio was 6.30 times, and the optimal subcultures were 5-6 generations. (5) Seedlings were transferred to 1/2 MS + 0.2 ~ 0.3 mg / L NAA, rooting rate was 100%; (6) Transplanting into the substrate of peat or garden soil and keeping warm and moisturizing, the survival rate was 92.30% and 88.00% .