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用TUNEL法对肝细胞肝癌、乳腺癌、胃腺癌以及结肠腺癌组织中的凋亡细胞进行形态学观察。主要采用附加蛋白酶K和微波预处理的TUNEL试剂盒(德国宝灵曼 公司和武汉博士德公司生产)对福尔吗啉固定、石蜡包埋的上述肿瘤组织进行TUNEL染色,荧光、光学显微镜观察凋亡细胞的形态特征。结果:TUNEL阳性信号位于胞核,呈环形、小圆形或颗粒状。TUNEL阳性细胞大多为凋亡的瘤细胞和肿瘤浸润的淋巴细胞。结论:TUNEL技术是目前原位观察凋亡的理想方法,特别适用于凋亡细胞的早期检测。
Apoptotic cells in hepatocellular carcinoma, breast cancer, gastric adenocarcinoma and colon adenocarcinoma were observed by TUNEL method. TUNEL staining was performed on formalin-fixed, paraffin-embedded tumor tissues using TUNEL kit with additional proteinase K and microwave pretreatment (manufactured by Bolinger and Bausch & Co., Germany), fluorescence, and light microscope observation. The morphological characteristics of dead cells. RESULTS: TUNEL-positive signals were located in the nucleus and were circular, small round or granular. Most of the TUNEL-positive cells were apoptotic tumor cells and tumor-infiltrating lymphocytes. Conclusion: TUNEL technique is the ideal method for in situ observation of apoptosis, especially for the early detection of apoptotic cells.