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1997年 4月 10～ 2 8日 ,在河北省石家庄市某高校发生一起成人急性腹泻的爆发流行 ,累积10 0 0多名大学生发病。经不连续聚丙烯酰胺凝胶 (PAGE)电泳检查腹泻病人粪便标本核酸图谱一致呈4 - 2 - 1- 1- 1- 1- 1排列的新轮状病毒 (新RV) 14份 ,阳性率占 4 7% (共检标本 30份 ) ,且未见其它带型的轮状病毒。提示该核酸图谱的轮状病毒是此次成人急性腹泻爆发流行的主要病原。用已知ADRV第 5及第 9基因片段末端引物进行逆转录 -聚合酶链反应 (RT -PCR) ,结果阳性对照ADRV的扩增结果都为阳性 ,而新RV的扩增结果都为阴性。这些结果表明该新RV不属于B组轮状病毒 ,是与成人腹泻轮状病毒 (ADRV)完全不同的新RV。非ADRV的轮状病毒引起成人腹泻爆发流行在河北省尚属首次。
From April 10 to April 8, 1997, an outbreak of adult acute diarrhea broke out in a university in Shijiazhuang, Hebei Province, accumulating more than 100 college students. The nucleic acid profiles of stool samples of diarrhea patients were consistent by discontinuous polyacrylamide gel electrophoresis (PAGE), and 14 new rotavirus (new RV) were arranged in 4-2-1-1-1-1 groups. The positive rate 47% (a total of 30 samples), and no other type of rotavirus. The rotavirus suggesting that the nucleic acid map is the major cause of the epidemic of acute diarrhea in adults. Reverse transcription-polymerase chain reaction (RT-PCR) was performed with the end primers of the 5 and 9 gene fragments of known ADRV. As a result, the positive control ADRV was all positive, and the result of the new RV was negative. These results indicate that the new RV does not belong to the group B rotavirus and is a completely new RV that differs from adult diarrhea rotavirus (ADRV). It is the first time that non-ADRV rotavirus causes the outbreak of adult diarrhea in Hebei Province.