论文部分内容阅读
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.Ubiquitin-conjugating enzyme E2 D2 is a protein that is encoded by the UBE2D2 gene.Here,we report a lamprey(La UBE2D2)gene which contained 441-bp open reading frame(ORF)encoding 147 amino acids with a typical UBC domain.Real-time PCR assay showed that the highest expression of the protein in adult lamprey was in the leukocytes,the lowest expression was in the skin,kidney and liver.The high conservation in amino acid sequence of the La UBE2D2protein with the UBE2D2s from Homo sapiens,Danio rerio,Oreochromis niloticus and Takifugu rubripes,implied that it had similar function with UBE2D2proteins from other species.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitin-conjugating enzyme E2 D2 is a protein that is encoded by the UBE2D2 gene. Here, we report a lamprey (La UBE2D2) gene which contained 441-bp open reading frame (ORF) encoding 147 amino acids with a typical UBC domain. Real-time PCR assay showed that the highest expression of the protein in adult lamprey was in the leukocytes, the lowest expression was in the skin, kidney and liver.The high conservation in amino acid sequence of the La UBE2D2protein with the UBE2D2s from Homo sapiens, Danio rerio, Oreochromis niloticus and Takifugu rubripes, implied that it had similar function with UBE2D2proteins from other species.