论文部分内容阅读
胡杨具有典型的异形叶性,是研究植物叶形发育的理想材料。但至今尚不清楚胡杨异形叶发生的分子调控机制。本研究以胡杨披针形和宽卵圆形幼叶为材料,利用qRT-PCR技术分析了参与叶基-顶轴发育的rot3和rot4基因、参与叶中-侧轴发育的an和spk1基因在胡杨异形叶发生中的表达模式,进一步分析了这些基因对胡杨异形叶发生的调控作用。发现rot3和spk1在披针形叶中的表达显著高于在宽卵圆形叶中表达,特别在叶形发育早期。而rot4和an则在宽卵圆形叶中的表达显著高于在披针形叶中表达,尤其是叶形发育早期。这暗示在胡杨叶形发育过程中,尤其是早期,rot3和spk1能促进胡杨叶形向披针形发育,而rot4和an则能促进胡杨叶形向宽卵圆形发育。同时也证明这4个预测基因在胡杨中是存在的,它们通过控制基-顶轴和中-侧轴极性建成调控胡杨异形叶的发生。
Populus euphratica has the typical shaped leaf, which is the ideal material to study the leaf shape development of plants. However, it is still unclear how the molecular regulation mechanism occurs in the deformed leaves of Populus euphratica. In this study, we used the young leaves of the lanceolate and broadly oval leaves as materials, and analyzed the rot3 and rot4 genes involved in leaf-top axis development by qRT-PCR. The an and spk1 genes involved in leaf mid- The expression pattern of the irregular-shaped leaves of Populus euphratica was analyzed, and the regulation effect of these genes on the occurrence of Abnormal-shaped leaves of Populus euphratica was further analyzed. The expression of rot3 and spk1 in the lanceolate leaves was found to be significantly higher than in the broadly ovoid leaves, especially in the early leaf-shaped development. However, the expression of rot4 and an in broadly ovoid leaves was significantly higher than that in lanceolate leaves, especially in early leaf development. This suggests that rot3 and spk1 can promote the leaf-shaped lanceolate development of Populus euphratica during leaf development, especially in the early stage, while rot4 and an can promote leaf-shaped broad-oval development of Populus euphratica. At the same time, it also proved that these four predicted genes exist in Populus euphratica. They regulate the development of Alhia lobata by controlling the polarity of the base-top and mid-lateral axes.