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目的:观察甲钴胺对糖尿病(DM)大鼠神经保护作用,探讨其可能的作用机制。方法:专选取60只健康Wistar大鼠,正常对照组(NG)20只,其余40只以链脲佐菌素(STZ)腹腔注射建立糖尿病大鼠模型。将DM大鼠随机分为糖尿病对照组(3M)20只,糖尿病甲钴胺治疗组(DMC)20只。2组控制血糖方法相同,8周后2组分别给予生理盐水、甲钴胺(1OOug/kg)隔日臀大肌肌注1次,连续4周,观察其坐骨神经传导速度(SNCV、MNCV)、cAMP、cGMP含量的影响及病理改变。结果:甲钴胺治疗组大鼠神经病变坐骨神经的传导速度明显提高,坐骨神经cAMP、cGMP含量明显增加。结论:甲钴胺对糖尿病大鼠神经功能有明显保护作用。
Objective: To observe the neuroprotective effect of mecobalamin on diabetic rats and its possible mechanism. Methods: Totally 60 healthy Wistar rats and 20 normal control rats were selected. The remaining 40 rabbits were randomly divided into three groups: diabetic rats model by intraperitoneal injection of streptozotocin (STZ). DM rats were randomly divided into diabetic control group (3M) 20, mecobalamin diabetic group (DMC) 20. 2 groups were given the same method of blood sugar control. After 8 weeks, the rats in the two groups were given normal saline respectively. Methylcobalamin (100ug / kg) was intramuscularly injected once every other day for 4 weeks. The sciatic nerve conduction velocity (SNCV, MNCV) , CGMP content and pathological changes. Results: The conduction velocity of sciatic nerves in mecobalamin group was significantly increased, and cAMP and cGMP contents in sciatic nerve were significantly increased. Conclusion: Mecobalamin significantly protects the neurological function of diabetic rats.