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目的观察候选抑癌基因SASH1与磷酸化的细胞外信号调节激酶1/2(p-ERK1/2)在胃腺癌中的表达、意义及二者的相互关系。方法免疫组织化学法检测152例胃腺癌组织及30例癌旁组织SASH1和p-ERK的表达。分析SASH1、p-ERK与胃癌临床病理学特征的关系及SASH1与p-ERK蛋白表达的相关性。结果 SASH1在胃癌组织中的阳性率显著低于癌旁组织(P<0.01),在胃低分化腺癌中的阳性率显著低于胃高、中分化腺癌(P<0.01)。SASH1阳性率在Ⅲ-Ⅳ期胃癌患者、有淋巴结转移患者及有远端转移患者分别显著低于Ⅰ-Ⅱ期患者、无淋巴结转移患者及无远端转移患者(P<0.05,P<0.01;P<0.05)。p-ERK1/2在胃癌组织中的阳性率显著高于癌旁组织(P<0.01),在胃低分化腺癌中的阳性率显著高于胃高、中分化腺癌(P<0.01)。p-ERK1/2阳性率在Ⅲ-Ⅳ期胃癌患者、有淋巴结转移患者及有远端转移患者分别显著高于Ⅰ-Ⅱ期患者、无淋巴结转移患者及无远端转移患者(P值分别为P<0.05;P<0.05;P<0.01)。SASH1与p-ERK在胃腺癌中的表达呈显著负相关(P<0.05)。结论SASH1蛋白可能通过调控ERK1/2信号通路抑制胃癌进展。
Objective To investigate the expression of SASH1 and phosphorylation of extracellular signal-regulated kinase 1/2 (p-ERK1 / 2) in gastric adenocarcinoma and its significance. Methods Immunohistochemistry was used to detect the expression of SASH1 and p-ERK in 152 cases of gastric adenocarcinoma and 30 cases of paracancerous tissues. The relationship between SASH1, p-ERK and the clinicopathological characteristics of gastric cancer and the correlation between SASH1 and p-ERK protein expression were analyzed. Results The positive rate of SASH1 in gastric cancer tissue was significantly lower than that in paracancerous tissues (P <0.01). The positive rate of SASH1 in gastric poorly differentiated adenocarcinoma was significantly lower than that in gastric adenocarcinoma and moderately differentiated adenocarcinoma (P <0.01). The positive rate of SASH1 was significantly lower in patients with stage Ⅲ-Ⅳ gastric cancer than those in patients with stage Ⅰ-Ⅱ, without lymph node metastasis and without distant metastasis (P <0.05, P <0.01; P <0.05). The positive rate of p-ERK1 / 2 in gastric cancer tissue was significantly higher than that in paracancerous tissues (P <0.01). The positive rate of p-ERK1 / 2 in gastric poorly differentiated adenocarcinoma was significantly higher than that in gastric adenocarcinoma and moderately differentiated adenocarcinoma (P <0.01). The positive rate of p-ERK1 / 2 in patients with stage III-IV gastric cancer, lymph node metastasis and distant metastasis were significantly higher than those in patients with stage I-II, without lymph node metastasis and without distant metastasis (P values were P <0.05; P <0.05; P <0.01). There was a significant negative correlation between SASH1 and p-ERK expression in gastric adenocarcinoma (P <0.05). Conclusion SASH1 protein may inhibit the progress of gastric cancer by regulating the ERK1 / 2 signaling pathway.