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为阐明小剂量胰岛素(1u.kg-1)与硒(180μg.kg-1)对糖尿病大鼠糖脂代谢及其对心肌细胞胰岛素信号分子表达的联合作用,采用One TouchⅡ血糖仪和血糖试纸测定血糖水平;微柱法测定糖化血红蛋白浓度;酶学法测定甘油三酯(TG)和总胆固醇(TC)含量;免疫印迹和免疫组化法检测心肌细胞中PI3K和GLUT4表达的变化。结果表明,联合用药组血糖和血脂明显下降(P<0.01);同时联合用药明显增加糖尿病大鼠心肌细胞上PI3K和GLUT4的表达(P<0.01)。整体动物实验结果表明:胰岛素和硒联合应用在降低血糖和血脂方面发挥了协同作用;胰岛素与硒联合应用通过改善糖尿病大鼠心肌细胞胰岛素信号转导通路中PI3K的表达,从而改善了GLUT4的移位障碍,最终改善心肌的能量代谢。
In order to elucidate the synergistic effect of low dose insulin (1u.kg-1) and selenium (180μg.kg-1) on glucose and lipid metabolism and insulin signaling molecule expression in diabetic rats, one-touch glucose meter and blood glucose test strip Blood glucose levels were measured. The concentration of glycosylated hemoglobin was determined by microcolumn method. The contents of triglyceride (TG) and total cholesterol (TC) were measured by enzyme-linked immunosorbent assay. The expressions of PI3K and GLUT4 were detected by Western blotting and immunohistochemistry. The results showed that the combined treatment group significantly decreased blood glucose and blood lipids (P <0.01); at the same time combination therapy significantly increased the expression of PI3K and GLUT4 (P <0.01) in diabetic rat myocardial cells. The results of the whole animal experiment showed that the combination of insulin and selenium exerted a synergistic effect in lowering blood glucose and blood lipid; the combination of insulin and selenium improved the GLUT4 shift by improving the PI3K expression in the insulin signal transduction pathway of cardiomyocytes in diabetic rats Disorders, the ultimate improvement of myocardial energy metabolism.