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我们在进行油茶、杉木等主要经济、用材树种杂种优势的细胞遗传基础研究中,设想通过染色体组型和显代的研究,探讨杂种优势的遗传机理以及优树早期鉴定和选择的理论根据。为此,从1980年3月开始,对普通油茶(Camellia oleifera)和板栗(Castanea mollissima BI)的染色体组型和显带进行了研究,结果如下: 一、材料和方法试验以普通油茶、板栗的根尖细胞为材料,采用低渗、去壁、火干、变性Giemsa染色等步骤处理,其技术流程是:
In the cytogenetic studies on heterosis such as Camellia oleifera, Cunninghamia lanceolata and other major economic and timber species, we envisaged to explore the genetic mechanism of heterosis and the theoretical basis for early identification and selection of elite trees through the study of genome and phenotype. Therefore, starting from March 1980, the genomics and banding of Camellia oleifera and Castanea mollissima BI were studied. The results are as follows: First, the materials and methods were tested with common camellia, chestnut Root tip cells for the material, using low permeability, to the wall, fire, denatured Giemsa staining and other steps, the technical process is: