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目的 探讨金黄色葡萄球菌 (简称金葡菌 )肠毒素B(SEB)单克隆抗体 (单抗 )对烫伤脓毒症大鼠急性肺损伤的保护作用。方法 雄性Wistar大鼠 5 6只随机分为正常对照组 (n =10 )、烫伤对照组 (n =10 )、烫伤后金葡菌感染组 (n =2 0 )和SEB单克隆抗体 (单抗 )拮抗组 (n =16)。测定肺组织SEB水平、髓过氧化物酶 (MPO )活性、肿瘤坏死因子 (TNF) α和干扰素 (IFN) γ表达的改变。结果 烫伤后金葡菌脓毒症动物肺脏SEB含量明显升高 ,伤后 2、6h分别为 66.85ng/g组织和 92 .46ng/g组织 ,与正常对照组 (14 .2 6ng/g组织 )和烫伤对照组 (17.3 2ng/g组织 )相比均为P <0 .0 1;同时 ,肺组织MPO活性显著增强 ,峰值可达 7.3 9U /g组织 ,与正常对照组 (2 .0 9U /g组织 )相比 P <0 .0 5。与之相应 ,肺组织MPO活性显著增强 (P <0 .0 5 )。同时 ,局部组织IFN γ和TNF α基因及其蛋白质表达明显上调 (P <0 .0 5 ) ,并与肺脏SEB含量呈高度正相关 (分别为 r=0 .92 0 7、P =0 .0 0 3 3和 r=0 .814 2、P =0 .0 2 5 8)。SEB单抗早期干预可有效降低肺组织中SEB含量 ,并显著抑制IFN γ和TNF α的产生 ,肺脏病理改变亦明显减轻。结论 SEB单抗干预可抑制IFN γ和TNF α等炎症介质的产生 ,从而显著减轻烫伤后金葡菌对机体的损害
Objective To investigate the protective effect of monoclonal antibodies against Staphylococcus aureus enterotoxin B (SEB) on acute lung injury in scalded sepsis rats. Methods Fifty-six male Wistar rats were randomly divided into normal control group (n = 10), burn control group (n = 10), staphylococcus aureus infection group (n = 20) and SEB monoclonal antibody ) Antagonist group (n = 16). The level of SEB, the activity of MPO and the expression of tumor necrosis factor (TNF) and interferon (IFN) γ in lung tissue were measured. Results SEB content in the lungs of staphylococcus aureus sepsis animals was significantly increased after burn. The levels of SEB were 66.85ng / g and 92.46ng / g at 2,6h after injury, respectively, which were significantly lower than those in the normal control group (14.26ng / g) (P <0.01). Compared with the scalded control group (17.32ng / g tissue), P <0.01; at the same time, the MPO activity in lung tissue was significantly increased, with a peak value of 7.39U / g. Compared with the normal control group g tissue) compared to P <0.05. Correspondingly, MPO activity in lung tissue was significantly increased (P <0.05). At the same time, the expression of IFNγ and TNFα mRNA and protein in local tissues were significantly up-regulated (P <0.05) and positively correlated with the level of SEB in lung tissues (r = 0.92 0 7, P = 0 .0 0 3 3 and r = 0 .814 2, P = 0 .0 2 5 8). The early intervention with SEB monoclonal antibody can effectively reduce the SEB content in lung tissue and significantly inhibit the production of IFNγ and TNFα, as well as significantly reduce the pathological changes of lung. Conclusion The SEB monoclonal antibody can inhibit the production of inflammatory mediators such as IFNγ and TNFα and thus significantly reduce the damage of Staphylococcus aureus to the body after scald