论文部分内容阅读
本文通过出生后7天和24个月两组大鼠晶状体与20%三硝基甲苯(TNT)甘油:水(8:2)混悬液体外培养,培养箱通5%CO2,恒温37℃,观察TNT对晶状体上皮细胞DNA的损伤程度,结果以反映单链DNA断裂强度的F630/F530比值表示。HPLC分析发现经体外TNT孵育的晶状体内含有TNT及其代谢产物;当共同培养96h后,F640/F530比值随着TNT剂量增加而增大,达到40μL(11.74μmol/L)时趋向平稳;当用TNT浓度为4.40μmol/L的相同剂量培养时,48小时后单链DNA断裂最为严重F630/F530比值为1.50,明显高子正常对照组(P<0.01);幼鼠单链DNA断裂程度显著高于老龄鼠(P<0.01)。
In this study, we cultured in vitro with 7% and 24 months postnatal rat lens and 20% trinitrotoluene (TNT) glycerol: water (8: 2) The degree of DNA damage in lens epithelial cells was observed by TNT. The results were expressed as F630 / F530 ratio, which reflects the breaking strength of single-stranded DNA. HPLC analysis showed that TNT and its metabolites were contained in the lens after in vitro TNT incubation. The ratio of F640 / F530 increased with the increase of TNT dosage when the cells were co-cultured for 96h, reaching a steady level of 40μL (11.74μmol / L) When cultured with the same dose of TNT at 4.40 μmol / L, the ratio of F630 / F530 was the highest at 48 hours, the ratio of F630 / F530 was significantly higher than that of the control group (P <0.01) The degree of strand DNA breakage was significantly higher than that of the aged mice (P <0.01).